| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Cathepsin B-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilized from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: |
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers. NA |
| Storage Instruction: |
Recombinant Cathepsin B can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability to cleave a flourogenic substrate, the measured activity is 3 nM/ug/min |
| Gene Symbol: |
CTSB |
| Gene ID: |
1508 |
| Uniprot ID: |
CATB_HUMAN |
| Immunogen Region: |
ARG18-ILE339 |
| Immunogen: |
Optimized DNA sequence encoding mature chain of human Cathepsin B (ARG18-ILE339) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum. |
| Function | Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis. |
| Protein Name | Cathepsin BApp SecretaseAppsCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain |
| Database Links | Reactome: R-HSA-1442490Reactome: R-HSA-1679131Reactome: R-HSA-2022090Reactome: R-HSA-2132295Reactome: R-HSA-6798695 |
| Cellular Localisation | LysosomeMelanosomeSecretedExtracellular SpaceApical Cell MembranePeripheral Membrane ProteinExtracellular SideIdentified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage IvLocalizes To The Lumen Of Thyroid Follicles And To The Apical Membrane Of Thyroid Epithelial Cells |
| Alternative Protein Names | Cathepsin B proteinApp Secretase proteinApps proteinCathepsin B1 Cleaved Into - Cathepsin B Light Chain - Cathepsin B Heavy Chain proteinCTSB proteinCPSB protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
