Human CASP4 (Caspase-4) Antibody Pair (STJAP00462)
General Information
| Short Description | This CASP4 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rat/Rat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | CASP4 |
| Gene ID | 837 |
| Uniprot ID | CASP4_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human CASP4 protien.Detection Immunogen: Recombinant Human CASP4 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | In response to activation signals, undergoes autoproteolytic cleavage and activation. (Microbial infection) ADP-riboxanation by S.flexneri OspC3 blocks CASP4 autoprocessing, preventing CASP4 activation and ability to recognize and cleave GSDMD, thereby thwarting the inflammasome/pyroptosis-mediated defense. |
| Function | Inflammatory caspase that acts as the effector of the non-canonical inflammasome by mediating lipopolysaccharide (LPS)-induced pyroptosis. Also indirectly activates the NLRP3 and NLRP6 inflammasomes. Acts as a thiol protease that cleaves a tetrapeptide after an Asp residue at position P1: catalyzes cleavage of CGAS, GSDMD and IL18. Effector of the non-canonical inflammasome independently of NLRP3 inflammasome and CASP1: the non-canonical inflammasome promotes pyroptosis through GSDMD cleavage without involving secretion of cytokine IL1B. In the non-canonical inflammasome, CASP4 is activated by direct binding to the lipid A moiety of LPS without the need of an upstream sensor. LPS-binding promotes CASP4 activation and CASP4-mediated cleavage of GSDMD and IL18, followed by IL18 secretion through the GSDMD pore, pyroptosis of infected cells and their extrusion into the gut lumen. Also indirectly promotes secretion of mature cytokines (IL1A and HMGB1) downstream of GSDMD-mediated pyroptosis via activation of the NLRP3 and NLRP6 inflammasomes. Involved in NLRP3-dependent CASP1 activation and IL1B secretion in response to non-canonical activators, such as UVB radiation or cholera enterotoxin. Involved in NLRP6 inflammasome-dependent activation in response to lipoteichoic acid (LTA), a cell-wall component of Gram-positive bacteria, which leads to CASP1 activation and IL1B secretion. Involved in LPS-induced IL6 secretion.this activity may not require caspase enzymatic activity. The non-canonical inflammasome is required for innate immunity to cytosolic, but not vacuolar, bacteria. Plays a crucial role in the restriction of S.typhimurium replication in colonic epithelial cells during infection. Activation of the non-canonical inflammasome in brain endothelial cells can lead to excessive pyroptosis, leading to blood-brain barrier breakdown. Pyroptosis limits bacterial replication, while cytokine secretion promotes the recruitment and activation of immune cells and triggers mucosal inflammation. May also act as an activator of adaptive immunity in dendritic cells, following activation by oxidized phospholipid 1-palmitoyl-2-arachidonoyl- sn-glycero-3-phosphorylcholine, an oxidized phospholipid (oxPAPC). Involved in cell death induced by endoplasmic reticulum stress and by treatment with cytotoxic APP peptides found in Alzheimer's patient brains. Cleavage of GSDMD is not strictly dependent on the consensus cleavage site but depends on an exosite interface on CASP4 that recognizes and binds the Gasdermin-D, C-terminal (GSDMD-CT) part. Catalyzes cleavage and maturation of IL18.IL18 processing also depends of the exosite interface on CASP4. In contrast, it does not directly process IL1B. During non-canonical inflammasome activation, cuts CGAS and may play a role in the regulation of antiviral innate immune activation. (Microbial infection) In response to the Td92 surface protein of the periodontal pathogen T.denticola, activated by cathepsin CTSG which leads to production and secretion of IL1A and pyroptosis of gingival fibroblasts. |
| Protein Name | Caspase-4Casp-4Ice And Ced-3 Homolog 2Ich-2Ice(Rel-IiMih1Protease Tx Cleaved Into - Caspase-4 Subunit P10 - Caspase-4 Subunit P20 |
| Database Links | Reactome: R-HSA-168638Reactome: R-HSA-5620971 |
| Cellular Localisation | CytoplasmCytosolEndoplasmic Reticulum MembranePeripheral Membrane ProteinCytoplasmic SideMitochondrionInflammasomeSecretedPredominantly Localizes To The Endoplasmic Reticulum (Er)Association With The Er Membrane Requires Tmem214Released In The Extracellular Milieu By Keratinocytes Following Uvb Irradiation |
| Alternative Antibody Pair Names | Caspase-4 antibody pairCasp-4 antibody pairIce And Ced-3 Homolog 2 antibody pairIch-2 antibody pairIce(Rel-Ii antibody pairMih1 antibody pairProtease Tx Cleaved Into - Caspase-4 Subunit P10 - Caspase-4 Subunit P20 antibody pairCASP4 antibody pairICH2 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CASP4 (Caspase-4) Antibody Pair (STJAP00462)
This antibody pair detects human Caspase-4 (CASP4), corresponding to UniProt accession P49662. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated, while the detection antibody is biotin-conjugated. Both are supplied at 0.5 mg/mL and were purified using Protein A or G.
This pair has been validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Compatible sample types include serum, plasma, and other biological fluids.
The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5. The detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5. Both components should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided.
Both the capture and detection antibodies were raised against recombinant human CASP4 protein. Both antibodies are rat-derived.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.