Human CASP3 (Caspase-3) Antibody Pair (STJAP00466)
General Information
| Short Description | This CASP3 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Goat/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | CASP3 |
| Gene ID | 836 |
| Uniprot ID | CASP3_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human CASP3 protien.Detection Immunogen: Recombinant Human CASP3 protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. Ubiquitinated by BIRC6.this activity is inhibited by DIABLO/SMAC. (Microbial infection) ADP-riboxanation by C.violaceum CopC blocks CASP3 processing, preventing CASP3 activation and ability to recognize and cleave substrates. |
| Function | Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively). Cleaves and inactivates interleukin-18 (IL18). Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Cleaves and inhibits serine/threonine-protein kinase AKT1 in response to oxidative stress. Acts as an inhibitor of type I interferon production during virus-induced apoptosis by mediating cleavage of antiviral proteins CGAS, IRF3 and MAVS, thereby preventing cytokine overproduction. Also involved in pyroptosis by mediating cleavage and activation of gasdermin-E (GSDME). Cleaves XRCC4 and phospholipid scramblase proteins XKR4, XKR8 and XKR9, leading to promote phosphatidylserine exposure on apoptotic cell surface. Cleaves BIRC6 following inhibition of BIRC6-caspase binding by DIABLO/SMAC. |
| Protein Name | Caspase-3Casp-3ApopainCysteine Protease Cpp32Cpp-32Protein YamaSrebp Cleavage Activity 1Sca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 |
| Database Links | Reactome: R-HSA-111459Reactome: R-HSA-111463Reactome: R-HSA-111464Reactome: R-HSA-111465Reactome: R-HSA-111469Reactome: R-HSA-140342Reactome: R-HSA-1474228Reactome: R-HSA-2028269Reactome: R-HSA-205025Reactome: R-HSA-211736Reactome: R-HSA-264870Reactome: R-HSA-351906Reactome: R-HSA-418889Reactome: R-HSA-449836Reactome: R-HSA-5620971 |
| Cellular Localisation | Cytoplasm |
| Alternative Antibody Pair Names | Caspase-3 antibody pairCasp-3 antibody pairApopain antibody pairCysteine Protease Cpp32 antibody pairCpp-32 antibody pairProtein Yama antibody pairSrebp Cleavage Activity 1 antibody pairSca-1 Cleaved Into - Caspase-3 Subunit P17 - Caspase-3 Subunit P12 antibody pairCASP3 antibody pairCPP32 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human CASP3 (Caspase-3) Antibody Pair (STJAP00466)
This antibody pair detects human Caspase-3 (CASP3, UniProt P42574) and has confirmed reactivity against human samples only. It is not validated for use with non-human species.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated; both are goat-raised, antigen affinity-purified, and provided at 0.5 mg/mL. The capture antibody is formulated in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), while the detection antibody is formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5).
This pair has been validated for use in ELISA only. No other assay formats are listed in the product specifications.
Validated sample types include serum, plasma, and other biological fluids. Use with sample types outside these categories has not been confirmed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human CASP3 protein. Both antibodies were subsequently purified by antigen affinity chromatography.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.