Human Carboxypeptidase A4 protein (Recombinant) (STJP000705)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding Human Carboxypeptidase A4 (GLY17-TYR421) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Carboxypeptidase A4-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Dilution Range | Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity | The activity was tested by the ability to cleave a fluorogenic substrate Ac-Phe-Thiaphe-OH in the presence of 5, 5 Dithio-bis. The specific activity was measured to be 3 nmole/ug/min NA |
Target Information
| Gene Symbol | CPA4 |
| Gene ID | 51200 |
| Uniprot ID | CBPA4_HUMAN |
| Immunogen | Optimized DNA sequence encoding Human Carboxypeptidase A4 (GLY17-TYR421) preprotein including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | GLY17-TYR421 |
Additional Info
| Tissue Specificity | Fetal expression in the adrenal gland, brain, heart, intestine, kidney, liver and lung. Except for fetal brain that shows no imprinting, expression was found preferentially from the maternal allele. |
| Function | Metalloprotease that cleaves hydrophobic C-terminal residues with a preference for -Phe, -Leu, -Ile, -Met, -Tyr and -Val. May function in peptide hormone and/or neuropeptide catabolism. |
| Protein Name | Carboxypeptidase A4Carboxypeptidase A3 |
| Database Links | |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Carboxypeptidase A4 proteinCarboxypeptidase A3 proteinCPA4 proteinCPA3 proteinUNQ694 proteinPRO1339 protein |
Information sourced from Uniprot.org