• Biotinylated Human CA125 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.
  • Immobilized Human MSLN, hFc Tag at 0. 5 Mu g/ml (100 Mu l/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 0. 35 Mu g/ml determined by ELISA.

Human CA125/MUC16 protein (Recombinant) (C-His-Avi) {Biotin} (STJP001725)

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STJP001725

€773.13 - €2,386.88
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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human CA125/MUC16-C-His-Avi protein was developed from hek293 and has a target region of C-His-Avi. For use in research applications.
Conjugation: Biotin
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Upon receipt centrifuge vial to ensure maximal product extraction, recommended: 20sec, 5K RPM
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C.
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg).
Gene Symbol: MUC16
Gene ID: 94025
Uniprot ID: MUC16_HUMAN
Immunogen Region: Gly12660-Met12923
Immunogen: DNA sequence encoding Human CA125/MUC16 including a C-His-Avi tag was expressed in HEK293 Cells. The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Post Translational Modifications Heavily O-glycosylated.expresses both type 1 and type 2 core glycans. Heavily N-glycosylated.expresses primarily high mannose and complex bisecting type N-linked glycans. May be phosphorylated. Phosphorylation of the intracellular C-terminal domain may induce proteolytic cleavage and the liberation of the extracellular domain into the extracellular space. May contain numerous disulfide bridges. Association of several molecules of the secreted form may occur through interchain disulfide bridges providing an extraordinarily large gel-like matrix in the extracellular space or in the lumen of secretory ducts.
Function Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces.
Protein Name Mucin-16
Muc-16
Ovarian Cancer-Related Tumor Marker Ca125
Ca-125
Ovarian Carcinoma Antigen Ca125
Database Links Reactome: R-HSA-5083625
Reactome: R-HSA-5083632
Reactome: R-HSA-5083636
Reactome: R-HSA-5621480
Reactome: R-HSA-913709
Reactome: R-HSA-977068
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Secreted
Extracellular Space
May Be Liberated Into The Extracellular Space Following The Phosphorylation Of The Intracellular C-Terminus Which Induces The Proteolytic Cleavage And Liberation Of The Extracellular Domain
Alternative Protein Names Mucin-16 protein
Muc-16 protein
Ovarian Cancer-Related Tumor Marker Ca125 protein
Ca-125 protein
Ovarian Carcinoma Antigen Ca125 protein
MUC16 protein
CA125 protein

Information sourced from Uniprot.org

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