Human C5a (Complement C5) Antibody Pair (STJAP00013)
General Information
| Short Description | This C5a antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | C5 |
| Gene ID | 727 |
| Uniprot ID | CO5_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human C5a protein.Detection Immunogen: Recombinant Human C5a protein. |
Additional Info
| Function | Precursor of the C5a anaphylatoxin and complement C5b components of the complement pathways, which consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. Activated downstream of classical, alternative, lectin and GZMK complement pathways. Complement C5b: Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. Complement C5b is generated following cleavage by C5 convertase and initiates formation of the MAC complex: C5b binds sequentially C6, C7, C8 and multiple copies of the pore-forming subunit C9. During MAC complex assembly, the C5b6 subcomplex, composed of complement C5b and C6, associates with the outer leaflet of target cell membrane, reducing the energy for membrane bending. C5a anaphylatoxin: Mediator of local inflammatory process released following cleavage by C5 convertase. Acts by binding to its receptor (C5AR1 or C5AR2), activating G protein-coupled receptor signaling and inducing a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes. C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation. |
| Protein Name | Complement C5C3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 4 Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5bComplement C5 Alpha' Chain |
| Database Links | Reactome: R-HSA-166665Reactome: R-HSA-174577Reactome: R-HSA-375276Reactome: R-HSA-418594Reactome: R-HSA-977606 |
| Cellular Localisation | SecretedComplement C5b: SecretedTarget Cell MembraneSecreted As Soluble ProteinInserts Into The Cell Membrane Of Target CellsC5a Anaphylatoxin: Secreted |
| Alternative Antibody Pair Names | Complement C5 antibody pairC3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 4 Cleaved Into - Complement C5 Beta Chain - Complement C5 Alpha Chain - C5a Anaphylatoxin - Complement C5b antibody pairComplement C5 Alpha' Chain antibody pairC5 antibody pairCPAMD4 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human C5a (Complement C5) Antibody Pair (STJAP00013)
This antibody pair detects human C5a, a cleavage fragment of complement component C5 (UniProt P01031). Reactivity is confirmed for human samples only.
The capture antibody is supplied unconjugated, and the detection antibody is biotin-conjugated. Both are mouse monoclonal antibodies supplied at 0.5 mg/mL, purified by Protein A or G, and formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair has been validated for use in ELISA. No other assay formats are specified for this product.
Both the capture and detection antibodies were raised against recombinant human C5a protein. No immunogen region mapping data is provided for either antibody.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.