Human beta Tubulin (beta Tubulin) Antibody Pair (STJAP00763)
General Information
| Short Description | This beta Tubulin antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R37-6Z-7/R79-7O-5 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | TUBB |
| Gene ID | 203068 |
| Uniprot ID | TBB5_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Ubiquitously expressed with highest levels in spleen, thymus and immature brain. |
| Post Translational Modifications | Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group. Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold. Glutamylation is also involved in cilia motility. Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable). Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules. |
| Function | Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin. |
| Protein Name | Tubulin Beta ChainTubulin Beta-5 Chain |
| Database Links | Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-6798695Reactome: R-HSA-8854518Reactome: R-HSA-9679191 |
| Cellular Localisation | CytoplasmCytoskeleton |
| Alternative Antibody Pair Names | Tubulin Beta Chain antibody pairTubulin Beta-5 Chain antibody pairTUBB antibody pairTUBB5 antibody pairOK antibody pairSW-cl.56 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human beta Tubulin (beta Tubulin) Antibody Pair (STJAP00763)
This antibody pair is designed to detect human beta Tubulin (UniProt P07437) and has confirmed reactivity in human samples only. No cross-reactivity data for other species is provided.
Both antibodies are rabbit monoclonal IgGs supplied unconjugated at 1 mg/mL, formulated in 0.01M PBS at pH 7.2, and affinity purified. The clone IDs are R37-6Z-7 and R79-7O-5 respectively.
This pair has been validated for use in ELISA. No other assay formats are listed as supported applications.
Optimal working dilutions are not predefined for this product and must be determined by the end user for their specific experimental conditions.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C, avoiding repeated freeze/thaw cycles.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.