Human Beta-EP (Beta-Endorphin) Competitive ELISA Kit (STJE0002322)

Name: Human Beta-EP (Beta-Endorphin) Competitive ELISA Kit (STJE0002322)
SKU: STJE0002322
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJE0002322

Current Stock:

🚚 Free UK Delivery on orders over £150

Quantity:

Processing The item has been added

Enquire For Bulk Order

✓ 1-Year Performance Guarantee | SDS & Datasheet Included | Expert Tech Support | Covered by St John's Laboratory Guarantee

General Information

Short Description	This Beta-EP Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications	ELISA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	2.5h
Detection	Colormetric

Target Information

Gene Symbol	POMC
Gene ID	5443
Uniprot ID	COLI_HUMAN
Specificity	This kit recognizes Human Beta-EP in samples. No significant cross-reactivity or interference between Human Beta-EP and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Post Translational Modifications	Specific enzymatic cleavages at paired basic residues yield the different active peptides. O-glycosylated.reducing sugar is probably N-acetylgalactosamine.
Function	Corticotropin: Stimulates the adrenal glands to release cortisol. Melanocyte-stimulating hormone alpha: Anorexigenic peptide. Increases the pigmentation of skin by increasing melanin production in melanocytes. Melanocyte-stimulating hormone beta: Increases the pigmentation of skin by increasing melanin production in melanocytes. Beta-endorphin: Endogenous orexigenic opiate. Met-enkephalin: Endogenous opiate.
Protein Name	Pro-Opiomelanocortin Pomc Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma Gamma-Msh - Potential Peptide - Corticotropin Adrenocorticotropic Hormone Acth - Melanocyte-Stimulating Hormone Alpha Alpha-Msh Melanotropin Alpha - Corticotropin-Like Intermediary Peptide Clip - Lipotropin Beta Beta-Lph - Lipotropin Gamma Gamma-Lph - Melanocyte-Stimulating Hormone Beta Beta-Msh Melanotropin Beta - Beta-Endorphin - Met-Enkephalin
Database Links	Reactome: R-HSA-111885 Reactome: R-HSA-193048 Reactome: R-HSA-194002 Reactome: R-HSA-202040 Reactome: R-HSA-209952 Reactome: R-HSA-211976 Reactome: R-HSA-375276 Reactome: R-HSA-418555 Reactome: R-HSA-418594 Reactome: R-HSA-5579031 Reactome: R-HSA-6785807 Reactome: R-HSA-9615017 Reactome: R-HSA-9856649
Cellular Localisation	Secreted Melanocyte-Stimulating Hormone Alpha And Beta-Endorphin Are Stored In Separate Granules In Hypothalamic Pomc Neurons Suggesting That Secretion May Be Under The Control Of Different Regulatory Mechanisms
Alternative ELISA Names	Pro-Opiomelanocortin ELISA kit Pomc ELISA kit Corticotropin-Lipotropin Cleaved Into - Npp - Melanotropin Gamma ELISA kit Gamma-Msh - Potential Peptide - Corticotropin ELISA kit Adrenocorticotropic Hormone ELISA kit Acth - Melanocyte-Stimulating Hormone Alpha ELISA kit Alpha-Msh ELISA kit Melanotropin Alpha - Corticotropin-Like Intermediary Peptide ELISA kit Clip - Lipotropin Beta ELISA kit Beta-Lph - Lipotropin Gamma ELISA kit Gamma-Lph - Melanocyte-Stimulating Hormone Beta ELISA kit Beta-Msh ELISA kit Melanotropin Beta - Beta-Endorphin - Met-Enkephalin ELISA kit POMC ELISA kit
Specificity	This kit recognizes β-EP in samples. No significant cross-reactivity or interference between β-EP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	90-107	97
EDTA plasma(n=8)	87-102	93
Cell culture media(n=8)	84-97	91

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	45.50	153.00	435.30	46.70	158.10	426.00
Standard deviation	2.90	7.50	20.00	2.80	6.60	13.60
CV (%)	6.37	4.90	4.59	6.00	4.17	3.19

Citations

View product citations for antibody STJE0002322 on CiteAb