Human BDNF protein (Active Recombinant) (C-His) (STJP001258)
SPECIFICATIONS
HostE.coli
ConjugationUnconjugated
ImmunogenRecombinant Human/Mouse/Rat BDNF Protein is produced by E. coli expression system. The target protein is expressed with sequence (His129-Arg247) of human BDNF (Accession #NP_733929.1.) fused with an initial Met at the N-terminus and a 6×His tag at th
General Information
| Short Description | Active Recombinant-Human BDNF-C-His protein was developed in e.coli using the region His129-Arg247. For use in research applications. |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | 1.Measured by its binding ability in a functional ELISA.Immobilized Recombinant Human BDNF at 1 Mu g/mL (100 Mu L/well) can bind BDNF Rabbit mAb with a linear range of 0.4-2.15ng/mL.|2.Measured by its binding ability in a functional ELISA.Immobilized |
| Determination Method | < 1.0 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | BDNF |
| Gene ID | 627 |
| Uniprot ID | BDNF_HUMAN |
| Immunogen | Recombinant Human/Mouse/Rat BDNF Protein is produced by E. coli expression system. The target protein is expressed with sequence (His129-Arg247) of human BDNF (Accession #NP_733929.1.) fused with an initial Met at the N-terminus and a 6×His tag at th |
| Immunogen Region | His129-Arg247 |
| Immunogen Sequence | HSDPARRGELSVCDSISEWV TAADKKTAVDMSGGTVTVLE KVPVSKGQLKQYFYETKCNP MGYTKEGCRGIDKRHWNSQC RTTQSYVRALTMDSKKRIGW RFIRIDTSCVCTLTIKRGR |
Additional Info
| Tissue Specificity | Detected in blood plasma and in saliva (at protein level). Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. |
| Post Translational Modifications | Neurotrophic factor BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG). |
| Function | Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Neurotrophic factor BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse. |
| Protein Name | Neurotrophic Factor Bdnf Precursor FormProbdnfAbrineurinBrain-Derived Neurotrophic Factor Cleaved Into - Neurotrophic Factor Bdnf |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-6811558Reactome: R-HSA-9022702Reactome: R-HSA-9024909Reactome: R-HSA-9026519Reactome: R-HSA-9026527Reactome: R-HSA-9028335Reactome: R-HSA-9028731Reactome: R-HSA-9032500Reactome: R-HSA-9032759Reactome: R-HSA-9032845Reactome: R-HSA-9768919 |
| Cellular Localisation | SecretedNeurotrophic Factor Bdnf Precursor Form: SecretedA Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf) |
| Alternative Protein Names | Neurotrophic Factor Bdnf Precursor Form proteinProbdnf proteinAbrineurin proteinBrain-Derived Neurotrophic Factor Cleaved Into - Neurotrophic Factor Bdnf proteinBDNF protein |
Information sourced from Uniprot.org