Human BDNF (Neurotrophic Factor Bdnf Precursor Form) Antibody Pair (STJAP00012)
General Information
| Short Description | This BDNF antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Polyclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | BDNF |
| Gene ID | 627 |
| Uniprot ID | BDNF_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human BDNF protein.Detection Immunogen: Recombinant Human BDNF protein. |
Additional Info
| Post Translational Modifications | Neurotrophic factor BDNF precursor form: N-glycosylated and glycosulfated, contrary to mature BDNF. Mature BDNF is produced by proteolytic removal of the propeptide, catalyzed by a FURIN family member. In addition, the precursor form is proteolytically cleaved within the propeptide, but this is not an obligatory intermediate for the production of mature BDNF. Can be converted into mature BDNF by plasmin (PLG). |
| Function | Important signaling molecule that activates signaling cascades downstream of NTRK2. During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Neurotrophic factor BDNF precursor form: Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse. |
| Protein Name | Neurotrophic Factor Bdnf Precursor FormProbdnfAbrineurinBrain-Derived Neurotrophic Factor Cleaved Into - Neurotrophic Factor Bdnf |
| Database Links | Reactome: R-HSA-1257604Reactome: R-HSA-2219530Reactome: R-HSA-6811558Reactome: R-HSA-9022702Reactome: R-HSA-9024909Reactome: R-HSA-9026519Reactome: R-HSA-9026527Reactome: R-HSA-9028335Reactome: R-HSA-9028731Reactome: R-HSA-9032500Reactome: R-HSA-9032759Reactome: R-HSA-9032845Reactome: R-HSA-9768919 |
| Cellular Localisation | SecretedNeurotrophic Factor Bdnf Precursor Form: SecretedA Proportion Of Bdnf Is Secreted As Immature Precursor (Probdnf) |
| Alternative Antibody Pair Names | Neurotrophic Factor Bdnf Precursor Form antibody pairProbdnf antibody pairAbrineurin antibody pairBrain-Derived Neurotrophic Factor Cleaved Into - Neurotrophic Factor Bdnf antibody pairBDNF antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human BDNF (Neurotrophic Factor Bdnf Precursor Form) Antibody Pair (STJAP00012)
This antibody pair detects human BDNF (Brain-Derived Neurotrophic Factor, precursor form), corresponding to UniProt accession P23560. Reactivity has been confirmed for human samples only.
The capture antibody is supplied unconjugated and the detection antibody is biotin-conjugated. Both are rabbit polyclonal antibodies purified by antigen affinity chromatography and supplied at 0.5 mg/mL in PBS containing 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. No other assay formats are listed in the product specifications.
Both the capture and detection antibodies were raised against recombinant human BDNF protein. No immunogen region or fragment is specified in the product details.
Both components should be stored at -20 degrees Celsius for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.