Human BCDIN3D protein (Recombinant) (His-Tag) (STJP017885)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human BCDIN3D-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 40% Glycerol, 1mM DTT, 2mM EDTA |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | BCDIN3D |
| Gene ID | 144233 |
| Uniprot ID | BN3D2_HUMAN |
| Accession Number | NP_859059 |
| Immunogen | Human |
| Immunogen Region | 1-292aa |
| Immunogen Sequence |
Additional Info
| Function | O-methyltransferase that specifically monomethylates 5'-monophosphate of cytoplasmic histidyl tRNA (tRNA(His)), acting as a capping enzyme by protecting tRNA(His) from cleavage by DICER1. Also able, with less efficiently, to methylate the 5' monophosphate of a subset of pre-miRNAs, acting as a negative regulator of miRNA processing. The 5' monophosphate of pre-miRNAs is recognized by DICER1 and is required for pre-miRNAs processing: methylation at this position reduces the processing of pre-miRNAs by DICER1. Was also reported to mediate dimethylation of pre-miR-145.however dimethylation cannot be reproduced by another group which observes a monomethylation of pre-miR-145. |
| Protein Name | Rna 5'-Monophosphate MethyltransferaseBcdin3 Domain-Containing Protein |
| Database Links | Reactome: R-HSA-203927 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Rna 5'-Monophosphate Methyltransferase proteinBcdin3 Domain-Containing Protein proteinBCDIN3D protein |
Information sourced from Uniprot.org