Human BCAM (BCAM) Antibody Pair (STJAP00953)
General Information
| Short Description | This BCAM antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL/0.5-4ug/mL |
| Dilution Range | 1:1000-1:10000 |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Detection | Colorimetric method;ELISA;Sandwich |
Target Information
| Gene Symbol | BCAM |
| Gene ID | 4059 |
| Uniprot ID | BCAM_HUMAN |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Epinephrine-stimulated phosphorylation of Ser-621 by PKA enhances adhesion to laminin. Ser-621 can also be phosphorylated by AKT1. |
| Function | Transmembrane glycoprotein that functions as both a receptor and an adhesion molecule playing a crucial role in cell adhesion, motility, migration and invasion. Extracellular domain enables binding to extracellular matrix proteins, such as laminin, integrin and other ligands while its intracellular domain interacts with cytoskeletal proteins like hemoglobin, facilitating cell signal transduction. Serves as a receptor for laminin alpha-5/LAMA5 to promote cell adhesion. Mechanistically, JAK2 induces BCAM phosphorylation and activates its adhesion to laminin by stimulating a Rap1/AKT signaling pathway in the absence of EPOR. |
| Protein Name | Basal Cell Adhesion MoleculeAuberger B AntigenB-Cam Cell Surface GlycoproteinF8/G253 AntigenLutheran AntigenLutheran Blood Group GlycoproteinCd Antigen Cd239 |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Pair Names | Basal Cell Adhesion Molecule antibody pairAuberger B Antigen antibody pairB-Cam Cell Surface Glycoprotein antibody pairF8/G253 Antigen antibody pairLutheran Antigen antibody pairLutheran Blood Group Glycoprotein antibody pairCd Antigen Cd239 antibody pairBCAM antibody pairLU antibody pairMSK19 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human BCAM (BCAM) Antibody Pair (STJAP00953)
This antibody pair detects human BCAM (Basal Cell Adhesion Molecule, UniProt P50895) and is validated for reactivity against human samples only.
The pair consists of two rabbit-raised antibodies: the capture antibody is unconjugated at a concentration of 0.5 mg/mL, and the detection antibody is biotin-conjugated at a concentration of 0.5 to 4 ug/mL.
This antibody pair is validated for use in sandwich ELISA with colorimetric detection. No other assay formats are specified for this product.
This antibody pair has been evaluated with serum, plasma, and other biological fluids. Suitability with other sample types is not specified in the product documentation.
A dilution range of 1:1000 to 1:10000 is indicated for this antibody pair. Optimal dilutions for a specific experimental setup should be determined empirically by the end user.
Both components should be stored at -20 degrees Celsius for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain product integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.