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Human BAG3 (BAG family molecular chaperone regulator 3) Sandwich ELISA Kit (STJE0002211)
Human BAG3 (BAG family molecular chaperone regulator 3) Sandwich ELISA Kit (STJE0002211)
General Information
Product Properties
Target Information
Additional Info
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General Information
Short Description This BAG3 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications ELISA
Reactivity Human
Sensitivity 0.09ng/mL
Detection Limit 0.16~10ng/mL
Note FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time 3.5h
Detection Colormetric
Additional Info
Protein Name Bag Family Molecular Chaperone Regulator 3Bag-3Bcl-2-Associated Athanogene 3Bcl-2-Binding Protein BisDocking Protein Cair-1 Database Links Reactome: R-HSA-3371453 Cellular Localisation NucleusCytoplasmColocalizes With Hsf1 To The Nucleus Upon Heat Stress Alternative ELISA Names Bag Family Molecular Chaperone Regulator 3 ELISA kitBag-3 ELISA kitBcl-2-Associated Athanogene 3 ELISA kitBcl-2-Binding Protein Bis ELISA kitDocking Protein Cair-1 ELISA kitBAG3 ELISA kitBIS ELISA kit Specificity This kit recognizes Human BAG3 in samples. No significant cross-reactivity or interference between Human BAG3 and analogues was observed. Reproducibility Both intra-CV and inter-CV are
Information sourced from Uniprot.org
Item Specifications Storage Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light) Stop Solution 1 vial, 10 mL 2-8°C Plate Sealer 5 pieces Manual 1 copy Certificate of Analysis 1 copy
Sample Type Range (%) Average Recovery (%) Serum(n=8) 85-95 90 EDTA plasma (n=8) 88-104 96 Cell culture media (n=8) 90-104 97
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision Sample 1.00 2.00 3.00 1.00 2.00 3.00 n 20.00 20.00 20.00 20.00 20.00 20.00 Mean (ng/mL) 0.49 1.19 4.73 0.47 1.10 5.17 Standard deviation 0.03 0.07 0.21 0.03 0.07 0.18 CV (%) 6.12 5.88 4.44 6.38 6.36 3.48
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