Human BACE1 (BACE1) Antibody Pair (STJAP00759)
General Information
| Short Description | This BACE1 antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | R77-9N-3/R58-6E-2 |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | Determined by end user |
| Formulation | 0.01M PBS, pH 7.2 |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | BACE1 |
| Gene ID | 23621 |
| Uniprot ID | BACE1_HUMAN |
| Immunogen | Recombinant protein |
Additional Info
| Tissue Specificity | Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. |
| Post Translational Modifications | N-Glycosylated. Addition of a bisecting N-acetylglucosamine by MGAT3 blocks lysosomal targeting, further degradation and is required for maintaining stability under stress conditions. Acetylated in the endoplasmic reticulum at Lys-126, Lys-275, Lys-279, Lys-285, Lys-299, Lys-300 and Lys-307. Acetylation by NAT8 and NAT8B is transient and deacetylation probably occurs in the Golgi. Acetylation regulates the maturation, the transport to the plasma membrane, the stability and the expression of the protein. Palmitoylation mediates lipid raft localization. Ubiquitinated at Lys-501, ubiquitination leads to lysosomal degradation. Monoubiquitinated and 'Lys-63'-linked polyubitinated. Deubiquitnated by USP8.inhibits lysosomal degradation. Phosphorylation at Ser-498 is required for interaction with GGA1 and retrograded transport from endosomal compartments to the trans-Golgi network. Non-phosphorylated BACE1 enters a direct recycling route to the cell surface. |
| Function | Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. Cleaves CHL1. |
| Protein Name | Beta-Secretase 1Aspartyl Protease 2Asp2Asp 2Beta-Site Amyloid Precursor Protein Cleaving Enzyme 1Beta-Site App Cleaving Enzyme 1Memapsin-2Membrane-Associated Aspartic Protease 2 |
| Database Links | Reactome: R-HSA-977225 |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinGolgi ApparatusTrans-Golgi NetworkEndoplasmic ReticulumEndosomeCell SurfaceCytoplasmic Vesicle MembraneMembrane RaftLysosomeLate EndosomeEarly EndosomeRecycling EndosomeCell ProjectionAxonDendritePredominantly Localized To The Later Golgi/Trans-Golgi Network (Tgn) And Minimally Detectable In The Early Golgi CompartmentsA Small Portion Is Also Found In The Endoplasmic ReticulumEndosomes And On The Cell SurfaceColocalization With App In Early Endosomes Is Due To Addition Of Bisecting N-Acetylglucosamine Which Blocks Targeting To Late Endosomes And LysosomesRetrogradly Transported From Endosomal Compartments To The Trans-Golgi Network In A Phosphorylation- And Gga1- Dependent Manner |
| Alternative Antibody Pair Names | Beta-Secretase 1 antibody pairAspartyl Protease 2 antibody pairAsp2 antibody pairAsp 2 antibody pairBeta-Site Amyloid Precursor Protein Cleaving Enzyme 1 antibody pairBeta-Site App Cleaving Enzyme 1 antibody pairMemapsin-2 antibody pairMembrane-Associated Aspartic Protease 2 antibody pairBACE1 antibody pairBACE antibody pairKIAA1149 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human BACE1 (BACE1) Antibody Pair (STJAP00759)
This antibody pair detects human BACE1 (Beta-secretase 1, UniProt P56817) and has confirmed reactivity with human samples only. Reactivity with other species has not been specified for this product.
Both antibodies in the pair are rabbit monoclonal IgGs, with clone IDs R77-9N-3 and R58-6E-2. Both are supplied unconjugated and affinity purified, allowing end users to assign capture and detection roles based on their own titration data.
This antibody pair is validated for use in ELISA. No other assay formats are listed for this product.
Both antibodies are supplied at 1 mg/mL, formulated in 0.01M PBS at pH 7.2. Neither antibody carries a conjugation or tag, so end users are responsible for any labeling steps required for their detection strategy.
Working dilutions are determined by the end user and are not provided in the product specification. Titration of both antibodies in the intended assay system is recommended to establish optimal capture and detection concentrations.
The antibodies should be stored at 4°C for short-term use. For long-term storage, aliquoting and keeping at -20°C is recommended. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.