Human B7-H4/VTCN1 protein (Recombinant) (C-His) (STJP000989)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human B7-H4/VTCN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe29-Ala258) of human B7-H4/B7S1/B7x (Accession #NP_078902.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human B7-H4/VTCN1-C-His protein was developed in hek293 cells using the region Phe29-Ala258. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by its binding ability in a functional ELISA.Immobilized Mouse B7-H4/VTCN1/VISTA at 1 Mu g/mL (100 Mu L/well) can bind B7-H4/VTCN1 Rabbit pAb with a linear range of 0.2-22 ng/mL. |
| Determination Method | < 0.1 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | VTCN1 |
| Gene ID | 79679 |
| Uniprot ID | VTCN1_HUMAN |
| Immunogen | Recombinant Human B7-H4/VTCN1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Phe29-Ala258) of human B7-H4/B7S1/B7x (Accession #NP_078902.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Phe29-Ala258 |
| Immunogen Sequence | FGISGRHSITVTTVASAGNI GEDGILSCTFEPDIKLSDIV IQWLKEGVLGLVHEFKEGKD ELSEQDEMFRGRTAVFADQV IVGNASLRLKNVQLTDAGTY KCYIITSKGKGNANLEYKTG AFSMPEVNVDYNASSETLRC EAPRWFPQPTVVWASQVDQG ANFSEVSNTSFELNSENVTM KVVSVLYNVTINNTYSCMIE NDIAKATGDIKVTESEIKRR SHLQLLNSKA |
Additional Info
| Tissue Specificity | Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis. |
| Post Translational Modifications | N-glycosylated. |
| Function | Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. |
| Protein Name | V-Set Domain-Containing T-Cell Activation Inhibitor 1B7 Homolog 4B7-H4B7h.5Immune Costimulatory Protein B7-H4Protein B7s1T-Cell Costimulatory Molecule B7x |
| Database Links | |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExpressed At The Cell SurfaceA Soluble Form Has Also Been Detected |
| Alternative Protein Names | V-Set Domain-Containing T-Cell Activation Inhibitor 1 proteinB7 Homolog 4 proteinB7-H4 proteinB7h.5 proteinImmune Costimulatory Protein B7-H4 proteinProtein B7s1 proteinT-Cell Costimulatory Molecule B7x proteinVTCN1 proteinB7H4 proteinUNQ659 proteinPRO1291 protein |
Information sourced from Uniprot.org