| Host: |
HEK293 |
| Note: |
STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human B7-H4-protein was developed from hek293. For use in research applications. |
| Conjugation: |
Unconjugated |
| Formulation: |
Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Dilution Range: |
Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers. |
| Storage Instruction: |
Can be stored in working aliquots at 2°C-8°C C for one month, or at-20°C to-70°C for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin: |
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA |
| Immunoreactivity: |
The activity was tested by the ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes. The calculated ED50 is < 0.7 ug/ml NA |
| Gene Symbol: |
VTCN1 |
| Gene ID: |
79679 |
| Uniprot ID: |
VTCN1_HUMAN |
| Immunogen Region: |
PHE29-ALA258 |
| Immunogen: |
Optimized DNA sequence encoding mature chain of Human B7-H4 (PHE29-ALA258) including a C-terminal His tag was expressed in HEK293 cells. NA |
| Tissue Specificity | Overexpressed in breast, ovarian, endometrial, renal cell (RCC) and non-small-cell lung cancers (NSCLC). Expressed on activated T- and B-cells, monocytes and dendritic cells, but not expressed in most normal tissues (at protein level). Widely expressed, including in kidney, liver, lung, ovary, placenta, spleen and testis. |
| Post Translational Modifications | N-glycosylated. |
| Function | Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation. |
| Protein Name | V-Set Domain-Containing T-Cell Activation Inhibitor 1B7 Homolog 4B7-H4B7h.5Immune Costimulatory Protein B7-H4Protein B7s1T-Cell Costimulatory Molecule B7x |
| Cellular Localisation | Cell MembraneSingle-Pass Type I Membrane ProteinExpressed At The Cell SurfaceA Soluble Form Has Also Been Detected |
| Alternative Protein Names | V-Set Domain-Containing T-Cell Activation Inhibitor 1 proteinB7 Homolog 4 proteinB7-H4 proteinB7h.5 proteinImmune Costimulatory Protein B7-H4 proteinProtein B7s1 proteinT-Cell Costimulatory Molecule B7x proteinVTCN1 proteinB7H4 proteinUNQ659 proteinPRO1291 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
