Human B3GAT3 protein (Recombinant) (His-Tag) (STJP018221)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human B3GAT3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | B3GAT3 |
| Gene ID | 26229 |
| Uniprot ID | B3GA3_HUMAN |
| Accession Number | NP_036332 |
| Immunogen | Human |
| Immunogen Region | 29-335aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSQPCDCLP PLRAAAEQLR QKDLRISQLQ AELRRPPPAP AQPPEPEALP TIYVVTPTYA RLVQKAELVR LSQTLSLVPR LHWLLVEDAE GPTPLVSGLL AASGLLFTHL VVLTPKAQRL REGEPGWVHP RGVEQRNKAL DWLRGRGGAV GGEKDPPPPG TQGVVYFADD DNTYSRELFE EMRWTRGVSV WPVGLVGGLR FEGPQVQD |
Additional Info
| Tissue Specificity | Ubiquitous (but weakly expressed in all tissues examined). |
| Post Translational Modifications | N-glycosylated. |
| Function | Glycosaminoglycans biosynthesis. Involved in forming the linkage tetrasaccharide present in heparan sulfate and chondroitin sulfate. Transfers a glucuronic acid moiety from the uridine diphosphate-glucuronic acid (UDP-GlcUA) to the common linkage region trisaccharide Gal-beta-1,3-Gal-beta-1,4-Xyl covalently bound to a Ser residue at the glycosaminylglycan attachment site of proteoglycans. Can also play a role in the biosynthesis of l2/HNK-1 carbohydrate epitope on glycoproteins. Shows strict specificity for Gal-beta-1,3-Gal-beta-1,4-Xyl, exhibiting negligible incorporation into other galactoside substrates including Galbeta1-3Gal beta1-O-benzyl, Galbeta1-4GlcNAc and Galbeta1-4Glc. Stimulates 2-phosphoxylose phosphatase activity of PXYLP1 in presence of uridine diphosphate-glucuronic acid (UDP-GlcUA) during completion of linkage region formation. |
| Protein Name | Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 3Beta-1 -3-Glucuronyltransferase 3Glucuronosyltransferase IGlcat-IUdp-Glcua -Gal Beta-1 -3-Gal-R GlucuronyltransferaseGlcuat-I |
| Database Links | Reactome: R-HSA-1971475Reactome: R-HSA-3560801 |
| Cellular Localisation | Golgi Apparatus MembraneSingle-Pass Type Ii Membrane ProteinGolgi ApparatusCis-Golgi Network |
| Alternative Protein Names | Galactosylgalactosylxylosylprotein 3-Beta-Glucuronosyltransferase 3 proteinBeta-1 -3-Glucuronyltransferase 3 proteinGlucuronosyltransferase I proteinGlcat-I proteinUdp-Glcua -Gal Beta-1 -3-Gal-R Glucuronyltransferase proteinGlcuat-I proteinB3GAT3 protein |
Information sourced from Uniprot.org