Human AZGP1 (Zinc-alpha-2-glycoprotein) Sandwich ELISA Kit (STJE0003045)

SPECIFICATIONS
STJE0003045
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General Information

Short DescriptionThis AZGP1 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
ApplicationsELISA
ReactivityHuman
Sensitivity0.94ng/mL
Detection Limit1.56~100ng/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionColormetric

Target Information

Gene SymbolAZGP1
Gene ID563
Uniprot IDZA2G_HUMAN
SpecificityThis kit recognizes Human AZGP1 in samples. No significant cross-reactivity or interference between Human AZGP1 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Post Translational Modifications N-glycosylated. N-glycan at Asn-128: Hex5HexNAc4.
Function Stimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
Protein Name Zinc-Alpha-2-Glycoprotein
Zn-Alpha-2-Gp
Zn-Alpha-2-Glycoprotein
Database Links Reactome: R-HSA-5223345
Cellular Localisation Secreted
Alternative ELISA Names Zinc-Alpha-2-Glycoprotein ELISA kit
Zn-Alpha-2-Gp ELISA kit
Zn-Alpha-2-Glycoprotein ELISA kit
AZGP1 ELISA kit
ZAG ELISA kit
ZNGP1 ELISA kit
Specificity This kit recognizes Human AZGP1 in samples. No significant cross-reactivity or interference between Human AZGP1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 92-106 98
EDTA plasma (n=8) 90-104 96
Cell culture media (n=8) 94-110 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 5.31 12.52 42.61 5.56 13.46 41.10
Standard deviation 0.34 0.56 1.91 0.38 0.72 1.85
CV (%) 6.40 4.47 4.48 6.83 5.35 4.50

Citations

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