| Host: |
HEK293 cells |
| Reactivity: |
Human |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Recombinant-Human Autotaxin/E-NPP2-C-His protein was developed from hek293 cells and has a target region of C-His. For use in research applications. |
| Formulation: |
Lyophilized from a 0.2 Mu m filtered solution of 20mM PB, 150mM NaCl, pH 7.4. |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
ENPP2 |
| Gene ID: |
5168 |
| Uniprot ID: |
ENPP2_HUMAN |
| Immunogen Region: |
Ala36-Ile863 |
| Immunogen: |
Recombinant Human Autotaxin/E-NPP2 Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Ala36-Ile863) of human ENPP-2 (Accession #AAH34961.1) fused with a 6xHis tag at the C-terminus. |
| Post Translational Modifications | N-glycosylation, but not furin-cleavage, plays a critical role on secretion and on lysoPLD activity. The interdomain disulfide bond between Cys-414 and Cys-806 is essential for catalytic activity. |
| Function | Hydrolyzes lysophospholipids to produce the signaling molecule lysophosphatidic acid (LPA) in extracellular fluids. Major substrate is lysophosphatidylcholine. Can also act on sphingosylphosphorylcholine producing sphingosine-1-phosphate, a modulator of cell motility. Can hydrolyze, in vitro, bis-pNPP, to some extent pNP-TMP, and barely ATP. Involved in several motility-related processes such as angiogenesis and neurite outgrowth. Acts as an angiogenic factor by stimulating migration of smooth muscle cells and microtubule formation. Stimulates migration of melanoma cells, probably via a pertussis toxin-sensitive G protein. May have a role in induction of parturition. Possible involvement in cell proliferation and adipose tissue development (Probable). Tumor cell motility-stimulating factor. Required for LPA production in activated platelets, cleaves the sn-1 lysophospholipids to generate sn-1 lysophosphatidic acids containing predominantly 18:2 and 20:4 fatty acids. Shows a preference for the sn-1 to the sn-2 isomer of 1-O-alkyl-sn-glycero-3-phosphocholine (lyso-PAF). |
| Protein Name | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2E-Npp 2AutotaxinExtracellular Lysophospholipase DLysopld |
| Database Links | Reactome: R-HSA-199220 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Ectonucleotide Pyrophosphatase/Phosphodiesterase Family Member 2 proteinE-Npp 2 proteinAutotaxin proteinExtracellular Lysophospholipase D proteinLysopld proteinENPP2 proteinATX proteinPDNP2 protein |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
