Human ATP4a (ATPase, H+/K+ Exchanging Alpha Polypeptide) Sandwich ELISA Kit (STJE0007390)
SPECIFICATIONS
General Information
| Short Description | This ATP4a Sandwich ELISA Kit is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human tissue homogenates, cell lysates or other biological fluids.. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.055ng/mL |
| Detection Limit | 0.156-10ng/mL |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Storage Instruction | Store the unopened kit in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit. |
| Assay Time | 4.5 hrs |
Target Information
| Gene Symbol | ATP4A |
| Gene ID | 495 |
| Uniprot ID | ATP4A_HUMAN |
| Sample Type | tissue homogenates, cell lysates or other biological fluids. |
Additional Info
| Tissue Specificity | Expressed in gastric parietal cells (at protein level). |
| Post Translational Modifications | |
| Function | The catalytic subunit of the gastric H(+)/K(+) ATPase pump which transports H(+) ions in exchange for K(+) ions across the apical membrane of parietal cells. Uses ATP as an energy source to pump H(+) ions to the gastric lumen while transporting K(+) ion from the lumen into the cell. Remarkably generates a million-fold proton gradient across the gastric parietal cell membrane, acidifying the gastric juice down to pH 1. Within a transport cycle, the transfer of a H(+) ion across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing (E1) to outward-facing state (E2). The release of the H(+) ion in the stomach lumen is followed by binding of K(+) ion converting the pump conformation back to the E1 state. |
| Protein Name | Potassium-Transporting Atpase Alpha Chain 1Gastric H(+/K(+ Atpase Subunit AlphaProton Pump |
| Database Links | Reactome: R-HSA-936837 |
| Cellular Localisation | Apical Cell MembraneMulti-Pass Membrane ProteinLocalized In The Apical Canalicular Membrane Of Parietal Cells |
| Alternative ELISA Names | Potassium-Transporting Atpase Alpha Chain 1 ELISA kitGastric H(+/K(+ Atpase Subunit Alpha ELISA kitProton Pump ELISA kitATP4A ELISA kit |
| output |
Information sourced from Uniprot.org