This ATP2C1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids..
Applications
ELISA
Reactivity
Human
Sensitivity
0.077ng/mL
Detection Limit
0.156-10ng/mL
Note
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Product Properties
Storage Instruction
The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.
Additional Info
Tissue Specificity
Found in most tissues except colon, thymus, spleen and leukocytes. Expressed in keratinocytes (at protein level).
Post Translational Modifications
Function
ATP-driven pump that supplies the Golgi apparatus with Ca(2+) and Mn(2+) ions, both essential cofactors for processing and trafficking of newly synthesized proteins in the secretory pathway. Within a catalytic cycle, acquires Ca(2+) or Mn(2+) ions on the cytoplasmic side of the membrane and delivers them to the lumenal side. The transfer of ions across the membrane is coupled to ATP hydrolysis and is associated with a transient phosphorylation that shifts the pump conformation from inward-facing to outward-facing state. Plays a primary role in the maintenance of Ca(2+) homeostasis in the trans-Golgi compartment with a functional impact on Golgi and post-Golgi protein sorting as well as a structural impact on cisternae morphology. Responsible for loading the Golgi stores with Ca(2+) ions in keratinocytes, contributing to keratinocyte differentiation and epidermis integrity. Participates in Ca(2+) and Mn(2+) ions uptake into the Golgi store of hippocampal neurons and regulates protein trafficking required for neural polarity. May also play a role in the maintenance of Ca(2+) and Mn(2+) homeostasis and signaling in the cytosol while preventing cytotoxicity.
Protein Name
Calcium-Transporting Atpase Type 2c Member 1Atpase 2c1Atp-Dependent Ca(2+ Pump Pmr1Ca(2+/Mn(2+-Atpase 2c1Secretory Pathway Ca(2+-Transporting Atpase Type 1Spca1
Golgi ApparatusTrans-Golgi Network MembraneMulti-Pass Membrane ProteinGolgi Stack MembraneDuring Neuron DifferentiationShifts From Juxtanuclear Golgi Position To Multiple Golgi Structures Distributed Over The Neural Soma With A Predominance In The Apical Dendritic Trunk
