Human ATN1 protein (Recombinant) (N-His) (STJP004926)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human ATN1-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Dilution Range | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| Formulation | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-80°C for twelve months from the date of receipt. |
Target Information
| Gene Symbol | ATN1 |
| Gene ID | 1822 |
| Uniprot ID | ATN1_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Asp879-Leu1190 |
Additional Info
| Post Translational Modifications | Phosphorylated in vitro by MAPK8/JNK1 on Ser-739. Mutant ATN1 sequences with expanded poly-Gln (polyQ) traits are more slowly phosphorylated. Proteolytically cleaved, probably in the nucleus, to produce two C-terminal fragments of 140 kDa (F1) and 125 kDa (F2) each containing poly-Gln (polyQ) tracts. F2 is produced by cleavage by caspases and is exported into the cytoplasm. In vitro, cleavage increases with an increase in the number of polyQ tracts. C-terminal proteolytic products appear to be the cause of cell toxicity. In vitro cleavage at Asp-109. |
| Function | Transcriptional corepressor. Recruits NR2E1 to repress transcription. Promotes vascular smooth cell (VSMC) migration and orientation. Corepressor of MTG8 transcriptional repression. Has some intrinsic repression activity which is independent of the number of poly-Gln (polyQ) repeats. |
| Protein Name | Atrophin-1Dentatorubral-Pallidoluysian Atrophy Protein |
| Database Links | Reactome: R-HSA-8943724 |
| Cellular Localisation | NucleusCytoplasmPerinuclear RegionCell JunctionShuttles Between Nucleus And CytoplasmColocalizes With Fat1 In The Perinuclear AreaAt Cell-Cell Junctions And Leading Edges Of CellsColocalizes With Mtg8 In Discrete Nuclear DotsProteolytic Fragment F1 Appears To Remain In NucleusFragment F2 Is Exported Into The CytoplasmFragment F2 From Mutant Sequences With Longer Poly-Gln (Polyq) Tracts Are Additionally Located To The Cytoplasmic Membrane And To Certain Organelles |
| Alternative Protein Names | Atrophin-1 proteinDentatorubral-Pallidoluysian Atrophy Protein proteinATN1 proteinD12S755E proteinDRPLA protein |
Information sourced from Uniprot.org