Human ASF1B protein (Recombinant) (His-Tag) (STJP017753)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human ASF1B-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol 0.15M NaCl, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ASF1B |
| Gene ID | 55723 |
| Uniprot ID | ASF1B_HUMAN |
| Accession Number | NP_060624.1 |
| Immunogen | Human |
| Immunogen Region | 1-202aa |
| Immunogen Sequence | MAKVSVLNVA VLENPSPFHS PFRFEISFEC SEALADDLEW KIIYVGSAES EEFDQILDSV LVGPVPAGRH MFVFQADAPN PSLIPETDAV GVTVVLITCT YHGQEFIRVG YYVNNEYLNP ELRENPPMKP DFSQLQRNIL ASNPRVTRFH INWDNNMDRL EAIETQDPSL GCGLPLNCTP IKGLGLPGCI PGLLPENSMD CILE |
Additional Info
| Tissue Specificity | Highly expressed in testis and at lower levels in colon, small intestine and thymus. |
| Post Translational Modifications | Phosphorylated by TLK1 and TLK2. |
| Function | Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Also involved in the nuclear import of the histone H3-H4 dimer together with importin-4 (IPO4): specifically recognizes and binds newly synthesized histones with the monomethylation of H3 'Lys-9' (H3K9me1) and diacetylation at 'Lys-5' and 'Lys-12' of H4 (H4K5K12ac) marks in the cytosol. Does not participate in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for gonad development. |
| Protein Name | Histone Chaperone Asf1bAnti-Silencing Function Protein 1 Homolog BHasf1Hasf1bCcg1-Interacting Factor A-IiCia-IiHcia-Ii |
| Database Links | |
| Cellular Localisation | NucleusCytoplasmCytosol |
| Alternative Protein Names | Histone Chaperone Asf1b proteinAnti-Silencing Function Protein 1 Homolog B proteinHasf1 proteinHasf1b proteinCcg1-Interacting Factor A-Ii proteinCia-Ii proteinHcia-Ii proteinASF1B protein |
Information sourced from Uniprot.org