Human Arylsulfatase G/ARSG protein (Recombinant) (His-Tag) (STJP017992)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Arylsulfatase G/ARSG-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Denatured |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARSG |
| Gene ID | 22901 |
| Uniprot ID | ARSG_HUMAN |
| Accession Number | NP_055775 |
| Immunogen | Human |
| Immunogen Region | 17-525aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSGFLYPLV DFCISGKTRG QKPNFVIILA DDMGWGDLGA NWAETKDTAN LDKMASEGMR FVDFHAAAST CSPSRASLLT GRLGLRNGVT RNFAVTSVGG LPLNETTLAE VLQQAGYVTG IIGKWHLGHH GSYHPNFRGF DYYFGIPYSH DMGCTDTPGY NHPPCPACPQ GDGPSRNLQR DCYTDVALPL YENLNIVEQP VNLSSLAQ |
Additional Info
| Tissue Specificity | Widely expressed, with very low expression in brain, lung, heart and skeletal muscle. |
| Post Translational Modifications | N-glycosylated. N-glycosylated with both high mannose and complex type sugars. The conversion to 3-oxoalanine (also known as C-formylglycine, FGly), of a serine or cysteine residue in prokaryotes and of a cysteine residue in eukaryotes, is critical for catalytic activity. The 63-kDa precursor undergoes proteolytic processing in two steps, yielding two fragments in the first step (apparent molecular masses of 44 and 18 kDa). In the second step, the 44-kDa fragment is processed further to the 34- and 10-kDa chains. The 10-kDa chain is a cleavage product of the 44-kDa fragment but linked to the 18-kDa chain through a disulfide bridge. |
| Function | Displays arylsulfatase activity at acidic pH towards artificial substrates, such as p-nitrocatechol sulfate and also, but with a lower activity towards p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate. Catalyzes the hydrolysis of the 3-sulfate groups of the N-sulfo-D-glucosamine 3-O-sulfate units of heparin. |
| Protein Name | Arylsulfatase GAsgN-Sulfoglucosamine-3-Sulfatase |
| Database Links | Reactome: R-HSA-1663150Reactome: R-HSA-9840310 |
| Cellular Localisation | LysosomeThe 63-Kda Precursor Protein Localizes To Pre-Lysosomal Compartments And Tightly Associates With Organelle MembranesMost Likely The Endoplasmic ReticulumIn ContrastProteolytically Processed Fragments Of 34-18- And 10-Kda Are Found In Lysosomal Fractions And Lose Their Membrane Association |
| Alternative Protein Names | Arylsulfatase G proteinAsg proteinN-Sulfoglucosamine-3-Sulfatase proteinARSG proteinKIAA1001 proteinUNQ839 proteinPRO1777 protein |
Information sourced from Uniprot.org