Human ARH3/ADPRS protein (Recombinant) (His-Tag) (STJP017774)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human ARH3/ADPRS-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.5 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 30% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ADPRS |
| Gene ID | 54936 |
| Uniprot ID | ADPRS_HUMAN |
| Accession Number | NP_060295 |
| Immunogen | Human |
| Immunogen Region | 1-363aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Ubiquitous. Expressed in skin fibroblasts. |
| Function | ADP-ribosylhydrolase that preferentially hydrolyzes the scissile alpha-O-linkage attached to the anomeric C1'' position of ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on serine and threonine, free poly(ADP-ribose) and O-acetyl-ADP-D-ribose. Specifically acts as a serine mono-ADP-ribosylhydrolase by mediating the removal of mono-ADP-ribose attached to serine residues on proteins, thereby playing a key role in DNA damage response. Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage. Does not hydrolyze ADP-ribosyl-arginine, -cysteine, -diphthamide, or -asparagine bonds. Also able to degrade protein free poly(ADP-ribose), which is synthesized in response to DNA damage: free poly(ADP-ribose) acts as a potent cell death signal and its degradation by ADPRHL2 protects cells from poly(ADP-ribose)-dependent cell death, a process named parthanatos. Also hydrolyzes free poly(ADP-ribose) in mitochondria. Specifically digests O-acetyl-ADP-D-ribose, a product of deacetylation reactions catalyzed by sirtuins. Specifically degrades 1''-O-acetyl-ADP-D-ribose isomer, rather than 2''-O-acetyl-ADP-D-ribose or 3''-O-acetyl-ADP-D-ribose isomers. |
| Protein Name | Adp-Ribosylhydrolase Arh3Adp-Ribose Glycohydrolase Arh3Adp-Ribosylhydrolase 3O-Acetyl-Adp-Ribose Deacetylase Arh3Poly(Adp-Ribose Glycohydrolase Arh3Protein Adp-Ribosylarginine Hydrolase-Like Protein 2Protein Adp-Ribosylserine Hydrolase |
| Database Links | Reactome: R-HSA-110362 |
| Cellular Localisation | NucleusCytoplasmChromosomeMitochondrion MatrixRecruited To Dna Lesion Regions Following Dna DamageAdp-D-Ribose-Recognition Is Required For Recruitment To Dna Damage Sites |
| Alternative Protein Names | Adp-Ribosylhydrolase Arh3 proteinAdp-Ribose Glycohydrolase Arh3 proteinAdp-Ribosylhydrolase 3 proteinO-Acetyl-Adp-Ribose Deacetylase Arh3 proteinPoly(Adp-Ribose Glycohydrolase Arh3 proteinProtein Adp-Ribosylarginine Hydrolase-Like Protein 2 proteinProtein Adp-Ribosylserine Hydrolase proteinADPRS proteinADPRHL2 proteinARH3 protein |
Information sourced from Uniprot.org