Human Arginase 1/ARG1 protein (Recombinant) (C-His) (STJP001020)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Arginase 1/ARG1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met 1-Lys 322 ) of human Arginase (Accession #NP_000036.2) fused with a 6×His tag at the C-terminus.
General Information
| Short Description | Recombinant-Human Arginase 1/ARG1-C-His protein was developed in hek293 cells using the region Met 1-Lys 322. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Measured by the production of urea during the hydrolysis of arginine. The specific activity is >27833 pmol/min/Mu g. |
| Determination Method | < 0.01 EU/Mu g of the protein by LAL method. |
Target Information
| Gene Symbol | ARG1 |
| Gene ID | 383 |
| Uniprot ID | ARGI1_HUMAN |
| Immunogen | Recombinant Human Arginase 1/ARG1 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met 1-Lys 322 ) of human Arginase (Accession #NP_000036.2) fused with a 6×His tag at the C-terminus. |
| Immunogen Region | Met 1-Lys 322 |
| Immunogen Sequence | MSAKSRTIGIIGAPFSKGQP RGGVEEGPTVLRKAGLLEKL KEQECDVKDYGDLPFADIPN DSPFQIVKNPRSVGKASEQL AGKVAEVKKNGRISLVLGGD HSLAIGSISGHARVHPDLGV IWVDAHTDINTPLTTTSGNL HGQPVSFLLKELKGKIPDVP GFSWVTPCISAKDIVYIGLR DVDPGEHYILKTLGIKYFSM TEVDRLGIGKVMEETLSYLL GRKKRPIHLSFDVDGLDPS |
Additional Info
| Tissue Specificity | Within the immune system initially reported to be selectively expressed in granulocytes (polymorphonuclear leukocytes PMNs). Also detected in macrophages mycobacterial granulomas. Expressed in group2 innate lymphoid cells (ILC2s) during lung disease. |
| Function | Key element of the urea cycle converting L-arginine to urea and L-ornithine, which is further metabolized into metabolites proline and polyamides that drive collagen synthesis and bioenergetic pathways critical for cell proliferation, respectively.the urea cycle takes place primarily in the liver and, to a lesser extent, in the kidneys. Functions in L-arginine homeostasis in nonhepatic tissues characterized by the competition between nitric oxide synthase (NOS) and arginase for the available intracellular substrate arginine. Arginine metabolism is a critical regulator of innate and adaptive immune responses. Involved in an antimicrobial effector pathway in polymorphonuclear granulocytes (PMN). Upon PMN cell death is liberated from the phagolysosome and depletes arginine in the microenvironment leading to suppressed T cell and natural killer (NK) cell proliferation and cytokine secretion. In group 2 innate lymphoid cells (ILC2s) promotes acute type 2 inflammation in the lung and is involved in optimal ILC2 proliferation but not survival. In humans, the immunological role in the monocytic/macrophage/dendritic cell (DC) lineage is unsure. |
| Protein Name | Arginase-1Liver-Type ArginaseType I Arginase |
| Database Links | Reactome: R-HSA-6798695Reactome: R-HSA-70635 |
| Cellular Localisation | CytoplasmCytoplasmic GranuleLocalized In Azurophil Granules Of Neutrophils |
| Alternative Protein Names | Arginase-1 proteinLiver-Type Arginase proteinType I Arginase proteinARG1 protein |
Information sourced from Uniprot.org