Human ARFIP1 protein (Recombinant) (His-Tag) (STJP018918)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human ARFIP1-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 0.25 mg/mL |
| Formulation | Liquid in phosphate-Buffered Saline (pH 7.4) containing 20% Glycerol, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ARFIP1 |
| Gene ID | 27236 |
| Uniprot ID | ARFP1_HUMAN |
| Accession Number | NP_001020766 |
| Immunogen | Human |
| Immunogen Region | 1-373aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MGSMAQESPK NSAAEIPVTS NGEVDDSREH SFNRDLKHSL PSGLGLSETQ ITSHGFDNTK EGVIEAGAFQ GSPAPPLPSV MSPSRVAASR LAQQGSDLIV PAGGQRTQTK SGPVILADEI KNPAMEKLEL VRKWSLNTYK CTRQIISEKL GRGSRTVDLE LEAQIDILRD NKKKYENILK LAQTLSTQLF QMVHTQRQLG DAFADLSL |
Additional Info
| Tissue Specificity | Ubiquitously expressed. Higher levels in liver, pancreas, placenta, skeletal muscle and heart. |
| Post Translational Modifications | Phosphorylated by PRKD1.phosphorylation delocalizes ARFIP1 from the Golgi and disrupts its ability to inhibit the activity of ADP-ribosylation factor, an important component of the vesicle scission machinery. |
| Function | Plays a role in controlling biogenesis of secretory granules at the trans-Golgi network. Mechanistically, binds ARF-GTP at the neck of a growing secretory granule precursor and forms a protective scaffold. Once the granule precursor has been completely loaded, active PRKD1 phosphorylates ARFIP1 and releases it from ARFs. In turn, ARFs induce fission. Through this mechanism, ensures proper secretory granule formation at the Golgi of pancreatic beta cells. |
| Protein Name | Arfaptin-1Adp-Ribosylation Factor-Interacting Protein 1 |
| Database Links | |
| Cellular Localisation | Golgi ApparatusTrans-Golgi Network Membrane |
| Alternative Protein Names | Arfaptin-1 proteinAdp-Ribosylation Factor-Interacting Protein 1 proteinARFIP1 protein |
Information sourced from Uniprot.org