• Human AREG (Amphiregulin) CLIA Kit (STJC0000161)

Human AREG (Amphiregulin) CLIA Kit (STJC0000161)

SKU:
STJC0000161

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Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 7.5pg/mL
Detection Limit: 12.5~800pg/mL
Short Description: This human AREG kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: AREG
Gene ID: 374
Uniprot ID: AREG_HUMAN
Specificity: This kit recognizes Human AR in samples. No significant cross-reactivity or interference between Human AR and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts.
Protein Name Amphiregulin
Ar
Colorectum Cell-Derived Growth Factor
Crdgf
Database Links Reactome: R-HSA-1257604
Reactome: R-HSA-177929
Reactome: R-HSA-179812
Reactome: R-HSA-180292
Reactome: R-HSA-180336
Reactome: R-HSA-182971
Reactome: R-HSA-204005
Reactome: R-HSA-212718
Reactome: R-HSA-2219530
Reactome: R-HSA-5638303
Reactome: R-HSA-5673001
Reactome: R-HSA-5694530
Reactome: R-HSA-6811558
Reactome: R-HSA-8856825
Reactome: R-HSA-8856828
Reactome: R-HSA-9009391
Reactome: R-HSA-9634638
Cellular Localisation Membrane
Single-Pass Membrane Protein
Alternative CLIA Names Amphiregulin CLIA kit
Ar CLIA kit
Colorectum Cell-Derived Growth Factor CLIA kit
Crdgf CLIA kit
AREG CLIA kit
AREGB CLIA kit
SDGF CLIA kit
Specificity This kit recognizes Human AR in samples. No significant cross-reactivity or interference between Human AR and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 87-101 93
EDTA plasma(n=8) 87-102 94
Cell culture media(n=8) 98-113 104

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 37.22 101.01 333.60 35.91 110.64 342.09
Standard deviation 4.17 11.55 35.33 4.31 10.98 29.80
CV (%) 11.20 11.43 10.59 12.00 9.92 8.71
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance