Human APOBEC3G protein (Recombinant) (N-His) (STJP015134)
SPECIFICATIONS
HostE.coli
ImmunogenHomo sapiens (Human)
General Information
| Short Description | Recombinant-Human APOBEC3G-N-His protein was developed from e.coli for the region N-His. For use in research applications. |
| Applications | ELISA/Immunogen/SDS-PAGE/WB |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Formulation | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. |
| Storage Instruction | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at-20 to-183°C for twelve months from the date of receipt. |
| Endotoxin | Please contact us for further information. |
Target Information
| Gene Symbol | APOBEC3G |
| Gene ID | 60489 |
| Uniprot ID | ABC3G_HUMAN |
| Immunogen | Homo sapiens (Human) |
| Immunogen Region | Met1-Asn384 |
Additional Info
| Post Translational Modifications | (Microbial infection) Following infection by HIV-1, ubiquitinated by a cullin-5-RING E3 ubiquitin-protein ligase complex (ECS complex) hijacked by the HIV-1 Vif protein, leading to its degradation. Deubiquitinated by USP49.leading to stabilization. Phosphorylation at Thr-32 reduces its binding to HIV-1 Vif and subsequent ubiquitination and degradation thus promoting its antiviral activity. |
| Function | DNA deaminase (cytidine deaminase) which acts as an inhibitor of retrovirus replication and retrotransposon mobility via deaminase-dependent and -independent mechanisms. Exhibits potent antiviral activity against Vif-deficient HIV-1. After the penetration of retroviral nucleocapsids into target cells of infection and the initiation of reverse transcription, it can induce the conversion of cytosine to uracil in the minus-sense single-strand viral DNA, leading to G-to-A hypermutations in the subsequent plus-strand viral DNA. The resultant detrimental levels of mutations in the proviral genome, along with a deamination-independent mechanism that works prior to the proviral integration, together exert efficient antiretroviral effects in infected target cells. Selectively targets single-stranded DNA and does not deaminate double-stranded DNA or single- or double-stranded RNA. Exhibits antiviral activity also against simian immunodeficiency viruses (SIVs), hepatitis B virus (HBV), equine infectious anemia virus (EIAV), xenotropic MuLV-related virus (XMRV) and simian foamy virus (SFV). May inhibit the mobility of LTR and non-LTR retrotransposons. |
| Protein Name | Dna Dc->Du-Editing Enzyme Apobec-3gApobec-Related Cytidine DeaminaseApobec-Related ProteinArcdApobec-Related Protein 9Arp-9Cem-15Cem15Deoxycytidine DeaminaseA3g |
| Database Links | Reactome: R-HSA-180585Reactome: R-HSA-180689 |
| Cellular Localisation | CytoplasmNucleusP-BodyMainly CytoplasmicSmall Amount Are Found In The NucleusDuring Hiv-1 InfectionVirion-Encapsidated In Absence Of Hiv-1 Vif |
| Alternative Protein Names | Dna Dc->Du-Editing Enzyme Apobec-3g proteinApobec-Related Cytidine Deaminase proteinApobec-Related Protein proteinArcd proteinApobec-Related Protein 9 proteinArp-9 proteinCem-15 proteinCem15 proteinDeoxycytidine Deaminase proteinA3g proteinAPOBEC3G proteinMDS019 protein |
Information sourced from Uniprot.org