Human ApoB (Apolipoprotein B) Sandwich ELISA Kit (STJE0019759)
SPECIFICATIONS
General Information
| Short Description | This ApoB Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids. |
| Applications | ELISA |
| Reactivity | Human |
| Sensitivity | 0.47ng/mL |
| Detection Limit | 0.78~50ng/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Colormetric |
Target Information
| Gene Symbol | APOB |
| Gene ID | 338 |
| Uniprot ID | APOB_HUMAN |
| Specificity | This kit recognizes Human ApoB in samples. No significant cross-reactivity or interference between Human ApoB and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Palmitoylated.structural requirement for proper assembly of the hydrophobic core of the lipoprotein particle. |
| Function | Apolipoprotein B is a major protein constituent of chylomicrons (apo B-48), LDL (apo B-100) and VLDL (apo B-100). Apo B-100 functions as a recognition signal for the cellular binding and internalization of LDL particles by the apoB/E receptor. |
| Protein Name | Apolipoprotein B-100Apo B-100 Cleaved Into - Apolipoprotein B-48Apo B-48 |
| Database Links | Reactome: R-HSA-202733Reactome: R-HSA-3000471Reactome: R-HSA-3000480Reactome: R-HSA-3000484Reactome: R-HSA-3000497Reactome: R-HSA-381426Reactome: R-HSA-432142Reactome: R-HSA-5686938Reactome: R-HSA-8856825Reactome: R-HSA-8856828Reactome: R-HSA-8866423Reactome: R-HSA-8957275Reactome: R-HSA-8963888Reactome: R-HSA-8963901Reactome: R-HSA-8964026Reactome: R-HSA-8964038Reactome: R-HSA-8964041Reactome: R-HSA-8964046Reactome: R-HSA-9707616Reactome: R-HSA-975634 |
| Cellular Localisation | CytoplasmSecretedLipid Droplet |
| Alternative ELISA Names | Apolipoprotein B-100 ELISA kitApo B-100 Cleaved Into - Apolipoprotein B-48 ELISA kitApo B-48 ELISA kitAPOB ELISA kit |
| Specificity | This kit recognizes Human ApoB in samples. No significant cross-reactivity or interference between Human ApoB and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent | 1 vial, 10 mL | 2-8℃ (Protect from light) |
| Stop Solution | 1 vial, 10 mL | 2-8°C |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 92-106 | 98 |
| EDTA plasma (n=8) | 92-108 | 99 |
| Cell culture media (n=8) | 91-106 | 98 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (ng/mL) | 2.27 | 6.02 | 20.35 | 2.21 | 5.90 | 18.84 |
| Standard deviation | 0.14 | 0.30 | 0.82 | 0.12 | 0.25 | 0.68 |
| CV (%) | 6.13 | 4.94 | 4.04 | 5.21 | 4.29 | 3.63 |