Human APOA5 (Apolipoprotein A-V) CLIA Kit for Quantitative Detection

Name: Human APOA5 (Apolipoprotein A-V) CLIA Kit for Quantitative Detection
SKU: STJC0000280
Availability: InStock

⇓ Datasheet ⇓ SDS

SPECIFICATIONS

STJC0000280

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General Information

Short Description	This human APOA5 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	9.38pg/mL
Detection Limit	15.63~1000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Target Information

Gene Symbol	APOA5
Gene ID	116519
Uniprot ID	APOA5_HUMAN
Specificity	This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Additional Info

Tissue Specificity	Liver and plasma.
Post Translational Modifications	Phosphorylated by FAM20C in the extracellular medium.
Function	Minor apolipoprotein mainly associated with HDL and to a lesser extent with VLDL. May also be associated with chylomicrons. Important determinant of plasma triglyceride (TG) levels by both being a potent stimulator of apo-CII lipoprotein lipase (LPL) TG hydrolysis and an inhibitor of the hepatic VLDL-TG production rate (without affecting the VLDL-apoB production rate). Activates poorly lecithin:cholesterol acyltransferase (LCAT) and does not enhance efflux of cholesterol from macrophages. Binds heparin.
Protein Name	Apolipoprotein A-V Apo-Av Apoa-V Apolipoprotein A5 Regeneration-Associated Protein 3
Database Links	Reactome: R-HSA-1989781 Reactome: R-HSA-381426 Reactome: R-HSA-8957275 Reactome: R-HSA-8963889 Reactome: R-HSA-8963901
Cellular Localisation	Secreted Early Endosome Late Endosome Golgi Apparatus Trans-Golgi Network In The Presence Of Sorl1 Internalized To Early Endosomes Sorted In A Retrograde Fashion To Late Endosomes From Which A Portion Is Sent To Lysosomes And Degradation Another Portion Is Sorted To The Trans-Golgi Network
Alternative CLIA Names	Apolipoprotein A-V CLIA kit Apo-Av CLIA kit Apoa-V CLIA kit Apolipoprotein A5 CLIA kit Regeneration-Associated Protein 3 CLIA kit APOA5 CLIA kit RAP3 CLIA kit UNQ411 CLIA kit PRO773 CLIA kit
Specificity	This kit recognizes Human ApoA5 in samples. No significant cross-reactivity or interference between Human ApoA5 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	89-104	95
EDTA plasma(n=8)	95-111	102
Cell culture media(n=8)	85-99	92

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	52.16	143.55	426.29	50.48	156.60	412.39
Standard deviation	6.22	15.75	27.37	4.74	13.56	42.72
CV (%)	11.92	10.97	6.42	9.39	8.66	10.36

Citations

View product citations for antibody STJC0000280 on CiteAb