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Human APOA2 (Apolipoprotein A-II) Sandwich ELISA Kit (STJE0004051)
Human APOA2 (Apolipoprotein A-II) Sandwich ELISA Kit (STJE0004051)
General Information
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General Information
Short Description This APOA2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human serum, plasma and other biological fluids.
Applications ELISA
Reactivity Human
Sensitivity 18.75ng/mL
Detection Limit 31.25~2000ng/mL
Note FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Product Properties
Storage Instruction If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time 3.5h
Detection Colormetric
Additional Info
Post Translational Modifications Phosphorylation sites are present in the extracellular medium. Apolipoprotein A-II is O-glycosylated. Function May stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. Protein Name Apolipoprotein A-IiApo-AiiApoa-IiApolipoprotein A2 Cleaved Into - Proapolipoprotein A-IiProapoa-Ii - Truncated Apolipoprotein A-IiApolipoprotein A-Ii(1-76 Database Links Reactome: R-HSA-1989781 Reactome: R-HSA-381426 Reactome: R-HSA-8957275 Reactome: R-HSA-8963888 Reactome: R-HSA-8963901 Reactome: R-HSA-975634 Cellular Localisation Secreted Alternative ELISA Names Apolipoprotein A-Ii ELISA kitApo-Aii ELISA kitApoa-Ii ELISA kitApolipoprotein A2 Cleaved Into - Proapolipoprotein A-Ii ELISA kitProapoa-Ii - Truncated Apolipoprotein A-Ii ELISA kitApolipoprotein A-Ii(1-76 ELISA kitAPOA2 ELISA kit Specificity This kit recognizes Human ApoA2 in samples. No significant cross-reactivity or interference between Human ApoA2 and analogues was observed. Reproducibility Both intra-CV and inter-CV are
Information sourced from Uniprot.org
Item Specifications Storage Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light) Stop Solution 1 vial, 10 mL 2-8°C Plate Sealer 5 pieces Manual 1 copy Certificate of Analysis 1 copy
Sample Type Range (%) Average Recovery (%) Serum(n=8) 89-99 94 EDTA plasma (n=8) 96-109 101 Cell culture media (n=8) 93-103 98
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision Sample 1.00 2.00 3.00 1.00 2.00 3.00 n 20.00 20.00 20.00 20.00 20.00 20.00 Mean (ng/mL) 96.14 224.95 844.46 86.28 245.63 844.50 Standard deviation 5.52 10.12 37.07 5.16 11.45 45.52 CV (%) 5.74 4.50 4.39 5.98 4.66 5.39
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