Human Angiopoietin-2 protein (Recombinant) (STJP000283)
SPECIFICATIONS
HostHEK293
ConjugationUnconjugated
ImmunogenOptimized DNA sequence encoding extracellular domain of Human Angiopoietin-2 including a C-terminal His tag was expressed in HEK293 cells. NA
General Information
| Short Description | Recombinant-Human Angiopoietin-2-protein was developed from hek293. For use in research applications. |
| Host | HEK293 |
| Note | STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from 0.2 Mu m filtered PBS solution, pH7.2, 5% Trehalose. |
| Storage Instruction | Can be stored in working aliquots at 2ยฐC-8ยฐC C for one month, or at-20ยฐC to-70ยฐC for 1 year. Avoid repeated freeze/thaw cycles. NA |
| Endotoxin | Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/ยตg (1EU/ยตg). NA |
| Immunoreactivity | The activity was tested by the ability of immobilized recombinant Human Tie2 to bind biotinylated recombinant human Angiopoietin-2 in ELISA NA |
Target Information
| Gene Symbol | ANGPT2 |
| Gene ID | 285 |
| Uniprot ID | ANGP2_HUMAN |
| Immunogen | Optimized DNA sequence encoding extracellular domain of Human Angiopoietin-2 including a C-terminal His tag was expressed in HEK293 cells. NA |
| Immunogen Region | ECD |
Additional Info
| Function | Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal. Involved in the regulation of lymphangiogenesis. |
| Protein Name | Angiopoietin-2Ang-2 |
| Database Links | Reactome: R-HSA-210993 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Angiopoietin-2 proteinAng-2 proteinANGPT2 protein |
Information sourced from Uniprot.org