Human Angiopoietin-1/ANG-1/ANGPT1 protein (Recombinant) (C-His) (STJP001989)
SPECIFICATIONS
HostHEK293 cells
ConjugationUnconjugated
ImmunogenRecombinant Human Angiopoietin-1/ANG-1/ANGPT1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg277-Phe498) of human Angiopoietin-1/ANG-1/ANGPT1 (Accession #NP_001137.2) fused with a 6×His tag at
General Information
| Short Description | Recombinant-Human Angiopoietin-1/ANG-1/ANGPT1-C-His protein was developed in hek293 cells using the region Arg277-Phe498. For use in research applications. |
| Host | HEK293 cells |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | < 0.1 EU/Mu g |
Target Information
| Gene Symbol | ANGPT1 |
| Gene ID | 284 |
| Uniprot ID | ANGP1_HUMAN |
| Immunogen | Recombinant Human Angiopoietin-1/ANG-1/ANGPT1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg277-Phe498) of human Angiopoietin-1/ANG-1/ANGPT1 (Accession #NP_001137.2) fused with a 6×His tag at |
| Immunogen Region | Arg277-Phe498 |
| Immunogen Sequence | KREEEKPFRDCADVYQAGFN KSGIYTIYINNMPEPKKVFC NMDVNGGGWTVIQHREDGSL DFQRGWKEYKMGFGNPSGEY WLGNEFIFAITSQRQYMLRI ELMDWEGNRAYSQYDRFHIG NEKQNYRLYLKGHTGTAGKQ SSLILHGADFSTKDADNDNC MCKCALMLTGGWWFDACGPS NLNGMFYTAGQNHGKLNGIK WHYFKGPSYSLRSTTMMIRP LDF |
Additional Info
| Function | Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. |
| Protein Name | Angiopoietin-1Ang-1 |
| Database Links | Reactome: R-HSA-210993Reactome: R-HSA-5673001 |
| Cellular Localisation | Secreted |
| Alternative Protein Names | Angiopoietin-1 proteinAng-1 proteinANGPT1 proteinKIAA0003 protein |
Information sourced from Uniprot.org