Human ANG (Angiogenin) Antibody Pair (STJAP00001)
General Information
| Short Description | This ANG antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Mouse/Goat |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Polyclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | ANG |
| Gene ID | 283 |
| Uniprot ID | ANGI_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human ANG protein.Detection Immunogen: Recombinant Human ANG protein. |
Additional Info
| Function | Secreted ribonuclease that can either promote or restrict cell proliferation of target cells, depending on the context. Endocytosed in target cells via its receptor PLXNB2 and translocates to the cytoplasm or nucleus. Under stress conditions, localizes to the cytoplasm and promotes the assembly of stress granules (SGs): specifically cleaves a subset of tRNAs within anticodon loops to produce tRNA-derived stress-induced fragments (tiRNAs), resulting in translation repression and inhibition of cell proliferation. tiRNas also prevent formation of apoptosome, thereby promoting cell survival. Preferentially cleaves RNAs between a pyrimidine and an adenosine residue, suggesting that it cleaves the anticodon loop of tRNA(Ala) (32-UUAGCAU-38) after positions 33 and 36. Cleaves a subset of tRNAs, including tRNA(Ala), tRNA(Glu), tRNA(Gly), tRNA(Lys), tRNA(Val), tRNA(His), tRNA(Asp) and tRNA(Sec). Under growth conditions and in differentiated cells, translocates to the nucleus and stimulates ribosomal RNA (rRNA) transcription, including that containing the initiation site sequences of 45S rRNA, thereby promoting cell growth and proliferation. Angiogenin induces vascularization of normal and malignant tissues via its ability to promote rRNA transcription. Involved in hematopoietic stem and progenitor cell (HSPC) growth and survival by promoting rRNA transcription in growth conditions and inhibiting translation in response to stress, respectively. Mediates the crosstalk between myeloid and intestinal epithelial cells to protect the intestinal epithelial barrier integrity: secreted by myeloid cells and promotes intestinal epithelial cells proliferation and survival. Also mediates osteoclast-endothelial cell crosstalk in growing bone: produced by osteoclasts and protects the neighboring vascular cells against senescence by promoting rRNA transcription. |
| Protein Name | AngiogeninRibonuclease 5Rnase 5 |
| Database Links | Reactome: R-HSA-418990Reactome: R-HSA-9708296 |
| Cellular Localisation | SecretedNucleusNucleolusCytoplasmStress GranuleThe Secreted Protein Is Rapidly Endocytosed By Target Cells Following Interaction With Plxnb2 Receptor And Translocated To The Cytoplasm And NucleusIn The NucleusAccumulates In The Nucleolus And Binds To Dna |
| Alternative Antibody Pair Names | Angiogenin antibody pairRibonuclease 5 antibody pairRnase 5 antibody pairANG antibody pairRNASE5 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human ANG (Angiogenin) Antibody Pair (STJAP00001)
This antibody pair detects Human Angiogenin (ANG), corresponding to UniProt accession P03950. Reactivity is confirmed for human samples only.
The capture antibody is an unconjugated mouse monoclonal antibody and the detection antibody is a biotin-conjugated goat polyclonal antibody. Both are supplied at 0.5 mg/mL, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This antibody pair is validated for use in ELISA. It is intended for research use only and is not validated for diagnostic or therapeutic applications.
Both the capture and detection antibodies were raised against recombinant Human ANG protein. The capture antibody was purified by Protein A or G, while the detection antibody was purified by antigen affinity.
Both components should be stored at -20 degrees C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.