Human Alpha-synuclein/SNCA protein (Recombinant) (C-His) (STJP002951)
SPECIFICATIONS
HostPichia
ConjugationUnconjugated
ImmunogenRecombinant Human Alpha-synuclein/SNCA Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala140) of human Alpha-synuclein/SNCA (Accession #NP_000336.1) fused with no additional amino acid.
General Information
| Short Description | Recombinant-Human Alpha-synuclein/SNCA-C-His protein was developed in pichia using the region Met1-Ala140. For use in research applications. |
| Host | Pichia |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated |
| Formulation | Lyophilised from a 0.22 Mu m filtered solution of PBS, pH 7.4. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Determination Method | <0.1 EU/Mu g |
Target Information
| Gene Symbol | SNCA |
| Gene ID | 6622 |
| Uniprot ID | SYUA_HUMAN |
| Immunogen | Recombinant Human Alpha-synuclein/SNCA Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala140) of human Alpha-synuclein/SNCA (Accession #NP_000336.1) fused with no additional amino acid. |
| Immunogen Region | Met1-Ala140 |
| Immunogen Sequence | GPYGANVEDSICCQDYIRHP LPSRLVKEFFWTSKSCRKPG VVLITVKNRDICADPRQVWV KKLLHKLS |
Additional Info
| Tissue Specificity | Highly expressed in presynaptic terminals in the central nervous system. Expressed principally in brain. |
| Post Translational Modifications | Phosphorylated, predominantly on serine residues. Phosphorylation by CK1 appears to occur on residues distinct from the residue phosphorylated by other kinases. Phosphorylation of Ser-129 is selective and extensive in synucleinopathy lesions. In vitro, phosphorylation at Ser-129 promoted insoluble fibril formation. Phosphorylated on Tyr-125 by a PTK2B-dependent pathway upon osmotic stress. Hallmark lesions of neurodegenerative synucleinopathies contain alpha-synuclein that is modified by nitration of tyrosine residues and possibly by dityrosine cross-linking to generated stable oligomers. Ubiquitinated. The predominant conjugate is the diubiquitinated form. Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure. |
| Function | Neuronal protein that plays several roles in synaptic activity such as regulation of synaptic vesicle trafficking and subsequent neurotransmitter release. Participates as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion and dilation of exocytotic fusion pores. Mechanistically, acts by increasing local Ca(2+) release from microdomains which is essential for the enhancement of ATP-induced exocytosis. Also acts as a molecular chaperone in its multimeric membrane-bound state, assisting in the folding of synaptic fusion components called SNAREs (Soluble NSF Attachment Protein REceptors) at presynaptic plasma membrane in conjunction with cysteine string protein-alpha/DNAJC5. This chaperone activity is important to sustain normal SNARE-complex assembly during aging. Also plays a role in the regulation of the dopamine neurotransmission by associating with the dopamine transporter (DAT1) and thereby modulating its activity. |
| Protein Name | Alpha-SynucleinNon-A Beta Component Of Ad AmyloidNon-A4 Component Of Amyloid PrecursorNacp |
| Database Links | Reactome: R-HSA-977225Reactome: R-HSA-9833482 |
| Cellular Localisation | CytoplasmMembraneNucleusSynapseSecretedCell ProjectionAxonMembrane-Bound In Dopaminergic NeuronsExpressed And Colocalized With Septin4 In Dopaminergic Axon TerminalsEspecially At The Varicosities |
| Alternative Protein Names | Alpha-Synuclein proteinNon-A Beta Component Of Ad Amyloid proteinNon-A4 Component Of Amyloid Precursor proteinNacp proteinSNCA proteinNACP proteinPARK1 protein |
Information sourced from Uniprot.org