Human Alpha 2-M (Alpha-2-Macroglobulin) Antibody Pair (STJAP00496)
General Information
| Short Description | This Alpha 2-M antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A or G.Detection Purification: Antigen Affinity. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | A2M |
| Gene ID | 2 |
| Uniprot ID | A2MG_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human Alpha 2-M protien.Detection Immunogen: Recombinant Human Alpha 2-M protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Function | Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region, a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase. |
| Protein Name | Alpha-2-MacroglobulinAlpha-2-MC3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-140837Reactome: R-HSA-1474228Reactome: R-HSA-8963896 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Alpha-2-Macroglobulin antibody pairAlpha-2-M antibody pairC3 And Pzp-Like Alpha-2-Macroglobulin Domain-Containing Protein 5 antibody pairA2M antibody pairCPAMD5 antibody pairFWP007 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Alpha 2-M (Alpha-2-Macroglobulin) Antibody Pair (STJAP00496)
This antibody pair detects human Alpha-2-Macroglobulin (Alpha 2-M, UniProt P01023) and is validated for human samples only. Both the capture and detection antibodies were raised against recombinant human Alpha 2-M protein.
Both antibodies are supplied at 0.5 mg/mL and are rabbit-raised. The capture antibody is unconjugated, formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.5, while the detection antibody is biotinylated and formulated in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol at pH 7.5.
This pair has been validated for use in ELISA. It is intended for sandwich ELISA-based detection of human Alpha 2-M in serum, plasma, and other biological fluids.
The capture antibody is purified by Protein A or G, and the detection antibody is purified by antigen affinity. The antigen affinity purification of the detection antibody is consistent with its use as a highly specific detection reagent in a sandwich ELISA format.
Both components should be stored at -20°C for long-term storage. Repeated freeze-thaw cycles should be avoided to maintain reagent integrity.
This pair is intended for use with human serum, plasma, and other biological fluids. It is for research use only and is not validated for diagnostic or therapeutic applications.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.