Human alpha 1 Fetoprotein (alpha 1 Fetoprotein) Antibody Pair (STJAP00669)
General Information
| Short Description | This alpha 1 Fetoprotein antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the. |
| Applications | ELISA |
| Host | Mouse/Mouse |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Clone ID | 3C11/10F12 |
| Isotype | IgG1/IgG2a |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | Affinity Purified |
| Dilution Range | ELISA 1:1000 |
| Formulation | Liquid in PBS containing 0.09% sodium azide as a preservative./Liquid in PBS containing 0.01% thimerosal as a preservative. |
| Storage Instruction | Store at 4°C short term. Aliquot and store at-20°C long term. Avoid freeze/thaw cycles. |
Target Information
| Gene Symbol | AFP |
| Gene ID | 174 |
| Uniprot ID | FETA_HUMAN |
| Immunogen | A synthesized peptide derived from AFP |
Additional Info
| Tissue Specificity | Plasma. Synthesized by the fetal liver and yolk sac. |
| Post Translational Modifications | Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence. Sulfated. |
| Function | Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties. |
| Protein Name | Alpha-FetoproteinAlpha-1-FetoproteinAlpha-Fetoglobulin |
| Database Links | Reactome: R-HSA-381426Reactome: R-HSA-8957275 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Alpha-Fetoprotein antibody pairAlpha-1-Fetoprotein antibody pairAlpha-Fetoglobulin antibody pairAFP antibody pairHPAFP antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human alpha 1 Fetoprotein (alpha 1 Fetoprotein) Antibody Pair (STJAP00669)
This antibody pair detects human alpha-1 fetoprotein (AFP), corresponding to UniProt entry P02771. Reactivity is confirmed for human samples only.
Both antibodies are mouse monoclonal IgGs supplied as affinity-purified liquids at 1 mg/mL. The capture antibody (clone 3C11, IgG1) is formulated in PBS with 0.09% sodium azide, while the detection antibody (clone 10F12, IgG2a) is formulated in PBS with 0.01% thimerosal. Neither antibody carries a conjugation or tag.
This pair is validated for use in ELISA, at a dilution of 1:1000. No other assay formats are listed in the product specifications.
Both antibodies should be stored at 4°C for short-term use. For long-term storage, aliquot and keep at -20°C. Repeated freeze/thaw cycles should be avoided to maintain antibody integrity.
Neither antibody is conjugated. The capture antibody (clone 3C11) is preserved with 0.09% sodium azide, and the detection antibody (clone 10F12) is preserved with 0.01% thimerosal. Researchers should account for these preservatives when designing downstream assay steps, particularly if enzyme-based detection systems are used.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.