Human Alpha 1-AT (Alpha-1-Antitrypsin) Antibody Pair (STJAP00233)
General Information
| Short Description | This Alpha 1-AT antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Clonality | Monoclonal/Monoclonal |
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Protein A.Detection Purification: Protein A. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4.Detection Buffer: PBS with 0.04% Proclin 300, 50% glycerol, pH 7.4. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
Target Information
| Gene Symbol | SERPINA1 |
| Gene ID | 5265 |
| Uniprot ID | A1AT_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human Alpha 1-AT protein.Detection Immunogen: Recombinant Human Alpha 1-AT protein. |
Additional Info
| Post Translational Modifications | N-glycosylated. Differential glycosylation produces a number of isoforms. N-linked glycan at Asn-107 is alternatively di-antennary, tri-antennary or tetra-antennary. The glycan at Asn-70 is di-antennary with trace amounts of tri-antennary. Glycan at Asn-271 is exclusively di-antennary. Structure of glycans at Asn-70 and Asn-271 is Hex5HexNAc4. The structure of the antennae is Neu5Ac(alpha1-6)Gal(beta1-4)GlcNAc attached to the core structure Man(alpha1-6)Man(alpha1-3)Man(beta1-4)GlcNAc(beta1-4)GlcNAc. Some antennae are fucosylated, which forms a Lewis-X determinant. Proteolytic processing may yield the truncated form that ranges from Asp-30 to Lys-418. (Microbial infection) Proteolytically processed by Staphylococcus aureus seryl, cysteinyl, and metallo-proteases. |
| Function | Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin. Short peptide from AAT: Reversible chymotrypsin inhibitor. It also inhibits elastase, but not trypsin. Its major physiological function is the protection of the lower respiratory tract against proteolytic destruction by human leukocyte elastase (HLE). |
| Protein Name | Alpha-1-AntitrypsinAlpha-1 Protease InhibitorAlpha-1-AntiproteinaseSerpin A1 Cleaved Into - Short Peptide From AatSpaat |
| Database Links | Reactome: R-HSA-114608Reactome: R-HSA-204005Reactome: R-HSA-381426Reactome: R-HSA-5694530Reactome: R-HSA-6798695Reactome: R-HSA-8957275 |
| Cellular Localisation | SecretedEndoplasmic ReticulumThe S And Z Allele Are Not Secreted Effectively And Accumulate Intracellularly In The Endoplasmic ReticulumShort Peptide From Aat: SecretedExtracellular SpaceExtracellular Matrix |
| Alternative Antibody Pair Names | Alpha-1-Antitrypsin antibody pairAlpha-1 Protease Inhibitor antibody pairAlpha-1-Antiproteinase antibody pairSerpin A1 Cleaved Into - Short Peptide From Aat antibody pairSpaat antibody pairSERPINA1 antibody pairAAT antibody pairPI antibody pairPRO0684 antibody pairPRO2209 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human Alpha 1-AT (Alpha-1-Antitrypsin) Antibody Pair (STJAP00233)
This antibody pair detects human Alpha-1-Antitrypsin (Alpha 1-AT), corresponding to UniProt entry P01009. Reactivity is confirmed for human samples only.
Both antibodies are rabbit monoclonal antibodies purified by Protein A and supplied at 0.5 mg/mL. The capture antibody is unconjugated, while the detection antibody is biotin-conjugated. Both are formulated in PBS with 0.04% Proclin 300 and 50% glycerol at pH 7.4.
This pair is validated for use in ELISA. No additional assay formats are listed in the product specifications.
Both antibodies were raised against recombinant human Alpha 1-AT protein. No further details regarding immunogen region or epitope mapping are provided in the current product specifications.
Both components should be stored at -20°C for long-term storage. Freeze-thaw cycles should be avoided to maintain antibody integrity.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.