SDS-PAGE gel showing recombinant human ALKBH3 protein 3µg stained with Coomassie blue

Human ALKBH3 protein (Recombinant) (His-Tag) (STJP017118)

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STJP017118
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Short Description :Recombinant-Human ALKBH3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
Applications:SDS-PAGE
Host:E.coli
Note:STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Concentration:1 mg/mL
Formulation:Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT, 0.1M NaCl.
Storage Instruction:For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.
Gene Symbol:ALKBH3
Gene ID:221120
Uniprot ID:ALKB3_HUMAN
Accession Number:NP_631917.1
Immunogen:Human
Immunogen Region:1-286aa
Immunogen Sequence:M EEKRRRARVQ GAWAAPVKSQ AIAQPATTAK SHLHQKPGQT WKNKEHHLSD REFVFKEPQQ VVRRAPEPRV IDREGVYEIS LSPTGVSRVC LYPGFVDVKE ADWILEQLCQ DVPWKQRTGI REDITYQQPR LTAWYGELPY TYSRITMEPN PHWHPVLRTL KNRIEENTGH TFNSLLCNLY RNEKDS
Tissue Specificity Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon.
Post Translational Modifications Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions.
Function Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron.
Protein Name Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3
Alkylated Dna Repair Protein Alkb Homolog 3
Habh3
Depc-1
Prostate Cancer Antigen 1
Database Links Reactome: R-HSA-112126
Cellular Localisation Nucleus
Cytoplasm
Colocalizes With Ascc2 And Ascc3 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna Damage
Predominantly Localizes To The Nucleus
Alternative Protein Names Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3 protein
Alkylated Dna Repair Protein Alkb Homolog 3 protein
Habh3 protein
Depc-1 protein
Prostate Cancer Antigen 1 protein
ALKBH3 protein
ABH3 protein
DEPC1 protein

Information sourced from Uniprot.org

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