Human ALKBH3 protein (Recombinant) (His-Tag) (STJP017118)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human ALKBH3-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 10% Glycerol, 2mM DTT, 0.1M NaCl. |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ALKBH3 |
| Gene ID | 221120 |
| Uniprot ID | ALKB3_HUMAN |
| Accession Number | NP_631917.1 |
| Immunogen | Human |
| Immunogen Region | 1-286aa |
| Immunogen Sequence |
Additional Info
| Tissue Specificity | Ubiquitous. Detected in heart, pancreas, skeletal muscle, thymus, testis, ovary, spleen, prostate, small intestine, peripheral blood leukocytes, urinary bladder and colon. |
| Post Translational Modifications | Ubiquitinated.undergoes 'Lys-48'-linked polyubiquitination. OTUD4 promotes USP7 and USP9X-dependent deubiquitination of 'Lys-48'-polyubiquitinated ALKBH3 promoting the repair of alkylated DNA lesions. |
| Function | Dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). Repairs alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. Has a strong preference for single-stranded DNA. Able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promotes DNA unwinding to generate single-stranded substrate needed for ALKBH3. Can repair exocyclic 3,N4-ethenocytosine adducs in single-stranded DNA. Also acts on RNA. Demethylates N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5'-untranslated regions (UTRs) and in the vicinity of start codons. Requires molecular oxygen, alpha-ketoglutarate and iron. |
| Protein Name | Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3Alkylated Dna Repair Protein Alkb Homolog 3Habh3Depc-1Prostate Cancer Antigen 1 |
| Database Links | Reactome: R-HSA-112126 |
| Cellular Localisation | NucleusCytoplasmColocalizes With Ascc2 And Ascc3 In Nuclear Foci When Cells Have Been Exposed To Alkylating Agents That Cause Dna DamagePredominantly Localizes To The Nucleus |
| Alternative Protein Names | Alpha-Ketoglutarate-Dependent Dioxygenase Alkb Homolog 3 proteinAlkylated Dna Repair Protein Alkb Homolog 3 proteinHabh3 proteinDepc-1 proteinProstate Cancer Antigen 1 proteinALKBH3 proteinABH3 proteinDEPC1 protein |
Information sourced from Uniprot.org