Human Aldo-keto reductase 1D1/AKR1D1 protein (Recombinant) (No-Tag) (STJP019265)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Aldo-keto reductase 1D1/AKR1D1-No-Tag protein was developed from e.coli and has a target region of No-Tag. For use in research applications. |
| Applications | SDS-PAGE |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.5) containing 1mM DTT, 0.1M NaCl, 10% Glycerol |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Endotoxin | < 1 EU per 1ug of protein (determined by LAL method) |
Target Information
| Gene Symbol | AKR1D1 |
| Gene ID | 6718 |
| Uniprot ID | AK1D1_HUMAN |
| Accession Number | NP_005980 |
| Immunogen | Human |
| Immunogen Region | 1-326aa |
| Immunogen Sequence | MDLSAASHRI PLSDGNSIPI IGLGTYSEPK STPKGACATS VKVAIDTGYR HIDGAYIYQN EHEVGEAIRE KIAEGKVRRE DIFYCGKLWA TNHVPEMVRP TLERTLRVLQ LDYVDLYIIE VPMAFKPGDE IYPRDENGKW LYHKSNLCAT WEAMEACKDA GLVKSLGVSN FNRRQLELIL NKPGLKHKPV SNQVECHPYF TQPKLLKFCQ QHDIVITAYS PLGTSRNP |
Additional Info
| Tissue Specificity | Highly expressed in liver. Expressed in testis and weakly in colon. |
| Function | Catalyzes the stereospecific NADPH-dependent reduction of the C4-C5 double bond of bile acid intermediates and steroid hormones carrying a delta(4)-3-one structure to yield an A/B cis-ring junction. This cis-configuration is crucial for bile acid biosynthesis and plays important roles in steroid metabolism. Capable of reducing a broad range of delta-(4)-3-ketosteroids from C18 (such as, 17beta-hydroxyestr-4-en-3-one) to C27 (such as, 7alpha-hydroxycholest-4-en-3-one). |
| Protein Name | Aldo-Keto Reductase Family 1 Member D13-Oxo-5-Beta-Steroid 4-DehydrogenaseDelta(4-3-Ketosteroid 5-Beta-ReductaseDelta(4-3-Oxosteroid 5-Beta-Reductase |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807 |
| Cellular Localisation | Cytoplasm |
| Alternative Protein Names | Aldo-Keto Reductase Family 1 Member D1 protein3-Oxo-5-Beta-Steroid 4-Dehydrogenase proteinDelta(4-3-Ketosteroid 5-Beta-Reductase proteinDelta(4-3-Oxosteroid 5-Beta-Reductase proteinAKR1D1 proteinSRD5B1 protein |
Information sourced from Uniprot.org