Human Aldo-keto Reductase 1C4/AKR1C4 protein (Recombinant) (His-Tag) (STJP017241)
SPECIFICATIONS
HostE.coli
ImmunogenHuman
General Information
| Short Description | Recombinant-Human Aldo-keto Reductase 1C4/AKR1C4-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications. |
| Applications | SDS-PAGE/Enzyme Activity |
| Host | E.coli |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Concentration | 1 mg/mL |
| Formulation | Liquid in 20mM Tris-HCl buffer (pH 8.0) containing 20% Glycerol, 0.1M NaCl, 1mM DTT |
| Storage Instruction | For short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles. |
| Immunoreactivity | Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C. |
Target Information
| Gene Symbol | AKR1C4 |
| Gene ID | 1109 |
| Uniprot ID | AK1C4_HUMAN |
| Accession Number | NP_001809 |
| Immunogen | Human |
| Immunogen Region | 1-323aa |
| Immunogen Sequence | MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKS |
Additional Info
| Tissue Specificity | Liver specific. |
| Post Translational Modifications | The N-terminus is blocked. |
| Function | Cytosolic aldo-keto reductase that catalyzes the NADH and NADPH-dependent reduction of ketosteroids to hydroxysteroids. Liver specific enzyme that acts as an NAD(P)(H)-dependent 3-, 17- and 20-ketosteroid reductase on the steroid nucleus and side chain. Displays the ability to catalyze both oxidation and reduction in vitro, but most probably acts as a reductase in vivo since the oxidase activity measured in vitro is inhibited by physiological concentration of NADPH. Acts preferentially as a 3-alpha-hydroxysteroid dehydrogenase (HSD) with a subsidiary 3-beta-HSD activity. Catalyzes efficiently the transformation of the potent androgen 5-alpha-dihydrotestosterone (5alpha-DHT or 17beta-hydroxy-5alpha-androstan-3-one) into the less active form, 5-alpha-androstan-3-alpha,17-beta-diol (3-alpha-diol). Catalyzes the reduction of estrone into 17beta-estradiol but with low efficiency. Metabolizes a broad spectrum of natural and synthetic therapeutic steroid and plays an important role in metabolism of androgens, estrogens, progestereone and conjugated steroids. Catalyzes the biotransformation of the pesticide chlordecone (kepone) to its corresponding alcohol leading to increased biliary excretion of the pesticide and concomitant reduction of its neurotoxicity since bile is the major excretory route. |
| Protein Name | Aldo-Keto Reductase Family 1 Member C4 3-Alpha-Hydroxysteroid Dehydrogenase Type I3-Alpha-Hsd13alpha-Hydroxysteroid 3-DehydrogenaseChlordecone ReductaseCdrDihydrodiol Dehydrogenase 4Dd-4Dd4Hakra |
| Database Links | Reactome: R-HSA-193368Reactome: R-HSA-193775Reactome: R-HSA-193807Reactome: R-HSA-975634 |
| Cellular Localisation | CytoplasmCytosol |
| Alternative Protein Names | Aldo-Keto Reductase Family 1 Member C4 protein3-Alpha-Hydroxysteroid Dehydrogenase Type I protein3-Alpha-Hsd1 protein3alpha-Hydroxysteroid 3-Dehydrogenase proteinChlordecone Reductase proteinCdr proteinDihydrodiol Dehydrogenase 4 proteinDd-4 proteinDd4 proteinHakra proteinAKR1C4 proteinCHDR protein |
Information sourced from Uniprot.org