Human AGT (Angiotensinogen) Antibody Pair (STJAP00513)
General Information
| Short Description | This AGT antibody pair contains complimentary capture and detection antibodies, which work together under the principals of the Sandwich ELISA. |
| Applications | ELISA |
| Host | Rabbit/Rabbit |
| Reactivity | Human |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Conjugation | Unconjugated/Biotin |
| Concentration | 0.5 mg/mL |
| Purification | Capture Purification: Antigen Affinity.Detection Purification: Protein A or G. |
| Formulation | Capture Buffer: PBS with 0.04% Proclin 300; 50% glycerol; pH 7.5.Detection Buffer: PBS with 0.04% Proclin 300; 1% protective protein; 50% glycerol; pH 7.5. |
| Storage Instruction | Store at-20°C for the long term, and avoid freeze-thaw cycles. |
| Determination Method | Colormetric |
Target Information
| Gene Symbol | AGT |
| Gene ID | 183 |
| Uniprot ID | ANGT_HUMAN |
| Immunogen | Capture immunogen: Recombinant Human AGT protien.Detection Immunogen: Recombinant Human AGT protien. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Post Translational Modifications | Beta-decarboxylation of Asp-25 in angiotensin-2, by mononuclear leukocytes produces alanine. The resulting peptide form, angiotensin-A, has the same affinity for the AT1 receptor as angiotensin-2, but a higher affinity for the AT2 receptor. In response to low blood pressure, the enzyme renin/REN cleaves angiotensinogen to produce angiotensin-1. Angiotensin-1 is a substrate of ACE (angiotensin converting enzyme) that removes a dipeptide to yield the physiologically active peptide angiotensin-2. Angiotensin-1 and angiotensin-2 can be further processed to generate angiotensin-3, angiotensin-4. Angiotensin 1-9 is cleaved from angiotensin-1 by ACE2 and can be further processed by ACE to produce angiotensin 1-7, angiotensin 1-5 and angiotensin 1-4. Angiotensin 1-7 has also been proposed to be cleaved from angiotensin-2 by ACE2 or from angiotensin-1 by MME (neprilysin). The disulfide bond is labile. Angiotensinogen is present in the circulation in a near 40:60 ratio with the oxidized disulfide-bonded form, which preferentially interacts with receptor-bound renin. |
| Function | Essential component of the renin-angiotensin system (RAS), a potent regulator of blood pressure, body fluid and electrolyte homeostasis. Angiotensin-2: Acts directly on vascular smooth muscle as a potent vasoconstrictor, affects cardiac contractility and heart rate through its action on the sympathetic nervous system, and alters renal sodium and water absorption through its ability to stimulate the zona glomerulosa cells of the adrenal cortex to synthesize and secrete aldosterone. Acts by binding to angiotensin receptors AGTR1 and AGTR2. Also binds the DEAR/FBXW7-AS1 receptor. Angiotensin-3: Stimulates aldosterone release. Angiotensin 1-7: Is a ligand for the G-protein coupled receptor MAS1. Has vasodilator and antidiuretic effects. Has an antithrombotic effect that involves MAS1-mediated release of nitric oxide from platelets. |
| Protein Name | AngiotensinogenSerpin A8 Cleaved Into - Angiotensin-1Angiotensin 1-10Angiotensin IAng I - Angiotensin-2Angiotensin 1-8Angiotensin IiAng Ii - Angiotensin-3Angiotensin 2-8Angiotensin IiiAng IiiDes-Asp1-Angiotensin Ii - Angiotensin-4Angiotensin 3-8Angiotensin IvAng Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 |
| Database Links | Reactome: R-HSA-1989781Reactome: R-HSA-2022377Reactome: R-HSA-375276Reactome: R-HSA-416476Reactome: R-HSA-418594 |
| Cellular Localisation | Secreted |
| Alternative Antibody Pair Names | Angiotensinogen antibody pairSerpin A8 Cleaved Into - Angiotensin-1 antibody pairAngiotensin 1-10 antibody pairAngiotensin I antibody pairAng I - Angiotensin-2 antibody pairAngiotensin 1-8 antibody pairAngiotensin Ii antibody pairAng Ii - Angiotensin-3 antibody pairAngiotensin 2-8 antibody pairAngiotensin Iii antibody pairAng Iii antibody pairDes-Asp1-Angiotensin Ii - Angiotensin-4 antibody pairAngiotensin 3-8 antibody pairAngiotensin Iv antibody pairAng Iv - Angiotensin 1-9 - Angiotensin 1-7 - Angiotensin 1-5 - Angiotensin 1-4 antibody pairAGT antibody pairSERPINA8 antibody pair |
Information sourced from Uniprot.org
Citations
Product Review
Frequently Asked Questions
Common questions about Human AGT (Angiotensinogen) Antibody Pair (STJAP00513)
This antibody pair detects human Angiotensinogen (AGT, UniProt P01019) and is validated for use with human samples only. Both the capture and detection antibodies were raised against recombinant human AGT protein.
The capture antibody is supplied unconjugated at 0.5 mg/mL in PBS with 0.04% Proclin 300 and 50% glycerol (pH 7.5), while the detection antibody is supplied biotinylated at 0.5 mg/mL in PBS with 0.04% Proclin 300, 1% protective protein, and 50% glycerol (pH 7.5). Both antibodies are rabbit-derived.
This pair is validated for use in sandwich ELISA. It is suitable for measuring human AGT in serum, plasma, and other biological fluids.
The capture antibody was purified by antigen affinity chromatography, while the detection antibody was purified using Protein A or G. These purification methods are standard approaches for producing antibodies intended for quantitative immunoassays.
Both antibodies should be stored at -20°C for long-term storage, and freeze-thaw cycles should be avoided to maintain activity. Aliquoting the reagents upon receipt is advisable to minimize repeated freeze-thaw events.
Orders typically arrive within 1 to 2 weeks, and often sooner depending on your location. If your timeline is critical, please contact us before ordering and we can advise on current lead times.
This product is shipped on gel ice packs to maintain integrity during transit. Priority shipping is used as standard to minimise time in transit.
A Certificate of Analysis (CoA) is provided with every order. If you do not receive one, please contact us and we will issue it promptly.