| Applications: |
CLIA |
| Reactivity: |
Human |
| Note: |
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
| Sensitivity: |
18.75pg/mL |
| Detection Limit: |
31.25~2000pg/mL |
| Short Description: |
This human AGGF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes. |
| Storage Instruction: |
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time: |
3.5h |
| Detection: |
Chemiluminescence |
| Gene Symbol: |
AGGF1 |
| Gene ID: |
55109 |
| Uniprot ID: |
AGGF1_HUMAN |
| Specificity: |
This kit recognizes Human AGGF1 in samples. No significant cross-reactivity or interference between Human AGGF1 and analogues was observed. |
| Sample Type: |
Serum, plasma and other biological fluids |
| Tissue Specificity | Widely expressed. Expressed in endothelial cells, vascular smooth muscle cells and osteoblasts. Expressed in umbilical vein endothelial cells and microvascular endothelial cells. |
| Function | Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion. |
| Protein Name | Angiogenic Factor With G Patch And Fha Domains 1Angiogenic Factor Vg5qHvg5qG Patch Domain-Containing Protein 7Vasculogenesis Gene On 5q Protein |
| Database Links | Reactome: R-HSA-6802952 |
| Cellular Localisation | CytoplasmSecretedCytoplasmic In Microvascular Endothelial CellsUpon AngiogenesisWhen Endothelial Cell Tube Formation Is InitiatedIt Is Secreted |
| Alternative CLIA Names | Angiogenic Factor With G Patch And Fha Domains 1 CLIA kitAngiogenic Factor Vg5q CLIA kitHvg5q CLIA kitG Patch Domain-Containing Protein 7 CLIA kitVasculogenesis Gene On 5q Protein CLIA kitAGGF1 CLIA kitGPATC7 CLIA kitGPATCH7 CLIA kitVG5Q CLIA kit |
| Specificity | This kit recognizes Human AGGF1 in samples. No significant cross-reactivity or interference between Human AGGF1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy | |
| Sample Type | Range (%) | Average Recovery (%) |
| Serum(n=8) | 88-103 | 95 |
| EDTA plasma(n=8) | 95-107 | 102 |
| Cell culture media(n=8) | 97-113 | 103 |
| | Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision |
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 107.42 | 208.77 | 827.42 | 113.14 | 209.48 | 818.85 |
| Standard deviation | 12.57 | 16.97 | 75.96 | 13.36 | 14.79 | 73.70 |
| CV (%) | 11.70 | 8.13 | 9.18 | 11.81 | 7.06 | 9.00 |
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
