Human AGGF1 (Angiogenic factor with G patch and FHA domains 1) CLIA Kit for Quantitative Detection

SPECIFICATIONS
STJC0000149
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General Information

Short DescriptionThis human AGGF1 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
ApplicationsCLIA
ReactivityHuman
Sensitivity18.75pg/mL
Detection Limit31.25~2000pg/mL
NoteFOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Product Properties

Storage InstructionIf unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time3.5h
DetectionChemiluminescence

Target Information

Gene SymbolAGGF1
Gene ID55109
Uniprot IDAGGF1_HUMAN
SpecificityThis kit recognizes Human AGGF1 in samples. No significant cross-reactivity or interference between Human AGGF1 and analogues was observed.
Sample TypeSerum, plasma and other biological fluids

Additional Info

Tissue Specificity Widely expressed. Expressed in endothelial cells, vascular smooth muscle cells and osteoblasts. Expressed in umbilical vein endothelial cells and microvascular endothelial cells.
Function Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion.
Protein Name Angiogenic Factor With G Patch And Fha Domains 1
Angiogenic Factor Vg5q
Hvg5q
G Patch Domain-Containing Protein 7
Vasculogenesis Gene On 5q Protein
Database Links Reactome: R-HSA-6802952
Cellular Localisation Cytoplasm
Secreted
Cytoplasmic In Microvascular Endothelial Cells
Upon Angiogenesis
When Endothelial Cell Tube Formation Is Initiated
It Is Secreted
Alternative CLIA Names Angiogenic Factor With G Patch And Fha Domains 1 CLIA kit
Angiogenic Factor Vg5q CLIA kit
Hvg5q CLIA kit
G Patch Domain-Containing Protein 7 CLIA kit
Vasculogenesis Gene On 5q Protein CLIA kit
AGGF1 CLIA kit
GPATC7 CLIA kit
GPATCH7 CLIA kit
VG5Q CLIA kit
Specificity This kit recognizes Human AGGF1 in samples. No significant cross-reactivity or interference between Human AGGF1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma(n=8) 95-107 102
Cell culture media(n=8) 97-113 103

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 107.42 208.77 827.42 113.14 209.48 818.85
Standard deviation 12.57 16.97 75.96 13.36 14.79 73.70
CV (%) 11.70 8.13 9.18 11.81 7.06 9.00

Citations

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