Human ADM (Pro-adrenomedullin) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human ADM kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 7.5pg/mL |
| Detection Limit | 12.5~800pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | ADM |
| Gene ID | 133 |
| Uniprot ID | ADML_HUMAN |
| Specificity | This kit recognizes Human ADM in samples. No significant cross-reactivity or interference between Human ADM and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Highest levels found in pheochromocytoma and adrenal medulla. Also found in lung, ventricle and kidney tissues. |
| Function | Adrenomedullin/ADM and proadrenomedullin N-20 terminal peptide/PAMP are peptide hormones that act as potent hypotensive and vasodilatator agents. Numerous actions have been reported most related to the physiologic control of fluid and electrolyte homeostasis. In the kidney, ADM is diuretic and natriuretic, and both ADM and PAMP inhibit aldosterone secretion by direct adrenal actions. In pituitary gland, both peptides at physiologically relevant doses inhibit basal ACTH secretion. Both peptides appear to act in brain and pituitary gland to facilitate the loss of plasma volume, actions which complement their hypotensive effects in blood vessels. Adrenomedullin: ADM function is mediated by the CALCRL-RAMP2 and CALCRL-RAMP3 receptor complexes with ADM showing the highest potency for the CALCRL-RAMP2 complex. |
| Protein Name | Pro-Adrenomedullin Cleaved Into - AdrenomedullinAm - Proadrenomedullin N-20 Terminal PeptideProam N-Terminal 20 PeptidePampProam-N20 |
| Database Links | Reactome: R-HSA-418555Reactome: R-HSA-419812Reactome: R-HSA-9856530 |
| Cellular Localisation | Secreted |
| Alternative CLIA Names | Pro-Adrenomedullin Cleaved Into - Adrenomedullin CLIA kitAm - Proadrenomedullin N-20 Terminal Peptide CLIA kitProam N-Terminal 20 Peptide CLIA kitPamp CLIA kitProam-N20 CLIA kitADM CLIA kitAM CLIA kit |
| Specificity | This kit recognizes Human ADM in samples. No significant cross-reactivity or interference between Human ADM and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 100-115 | 108 |
| EDTA plasma(n=8) | 86-101 | 92 |
| Cell culture media(n=8) | 90-104 | 95 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 43.82 | 84.72 | 300.61 | 47.63 | 80.10 | 314.32 |
| Standard deviation | 4.60 | 7.48 | 21.01 | 5.59 | 7.42 | 31.68 |
| CV (%) | 10.50 | 8.83 | 6.99 | 11.74 | 9.26 | 10.08 |