Human ACTN3 (Alpha-actinin-3) CLIA Kit for Quantitative Detection
SPECIFICATIONS
General Information
| Short Description | This human ACTN3 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids. |
| Applications | CLIA |
| Reactivity | Human |
| Sensitivity | 46.88pg/mL |
| Detection Limit | 78.13~5000pg/mL |
| Note | FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS. |
Product Properties
| Storage Instruction | If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual. |
| Assay Time | 3.5h |
| Detection | Chemiluminescence |
Target Information
| Gene Symbol | ACTN3 |
| Gene ID | 89 |
| Uniprot ID | ACTN3_HUMAN |
| Specificity | This kit recognizes Human ACTN3 in samples. No significant cross-reactivity or interference between Human ACTN3 and analogues was observed. |
| Sample Type | Serum, plasma and other biological fluids |
Additional Info
| Tissue Specificity | Expression restricted to fast (type 2) skeletal muscle fibers (at protein level). |
| Function | F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. |
| Protein Name | Alpha-Actinin-3Alpha-Actinin Skeletal Muscle Isoform 3F-Actin Cross-Linking Protein |
| Database Links | Reactome: R-HSA-373753Reactome: R-HSA-390522 |
| Cellular Localisation | |
| Alternative CLIA Names | Alpha-Actinin-3 CLIA kitAlpha-Actinin Skeletal Muscle Isoform 3 CLIA kitF-Actin Cross-Linking Protein CLIA kitACTN3 CLIA kit |
| Specificity | This kit recognizes Human ACTN3 in samples. No significant cross-reactivity or interference between Human ACTN3 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are |
Information sourced from Uniprot.org
| Item | Specifications | Storage |
|---|---|---|
| Micro CLIA Plate (Dismountable) | 96T: 8 wells ×12 strips strips | -20℃, 6 months |
| Reference Standard | 96T: 2 vials 48T: 1 vial | -20℃, 6 months |
| Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃, 6 months |
| Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL 60 μL | -20℃ (Protect from light), 6 months |
| Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
| Biotinylated Detection Ab Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| HRP Conjugate Diluent | 1 vial, 14 mL | 2-8°C, 6 months |
| Concentrated Wash Buffer (25×) | 1 vial, 30 mL | 2-8°C, 6 months |
| Substrate Reagent A | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Substrate Reagent B | 1 vial, 5 mL | 2-8℃ (Protect from light) |
| Plate Sealer | 5 pieces | |
| Manual | 1 copy | |
| Certificate of Analysis | 1 copy |
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 96-108 | 103 |
| EDTA plasma(n=8) | 97-109 | 103 |
| Cell culture media(n=8) | 90-103 | 97 |
| Intra-assay Precision | Intra-assay Precision | Intra-assay Precision | Inter-assay Precision | Inter-assay Precision | Inter-assay Precision | |
|---|---|---|---|---|---|---|
| Sample | 1.00 | 2.00 | 3.00 | 1.00 | 2.00 | 3.00 |
| n | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 | 20.00 |
| Mean (pg/mL) | 253.98 | 559.86 | 2190.94 | 267.68 | 574.35 | 2080.57 |
| Standard deviation | 22.05 | 58.56 | 153.80 | 27.25 | 43.82 | 214.92 |
| CV (%) | 8.68 | 10.46 | 7.02 | 10.18 | 7.63 | 10.33 |