Human ACTN2 (Alpha-actinin-2) CLIA Kit for Quantitative Detection

Name: Human ACTN2 (Alpha-actinin-2) CLIA Kit for Quantitative Detection
SKU: STJC0000167
Availability: InStock

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SPECIFICATIONS

STJC0000167

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Short Description	This human ACTN2 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes in serum, plasma and other biological fluids.
Applications	CLIA
Reactivity	Human
Sensitivity	46.88pg/mL
Detection Limit	78.13~5000pg/mL
Note	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.

Storage Instruction	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time	3.5h
Detection	Chemiluminescence

Gene Symbol	ACTN2
Gene ID	88
Uniprot ID	ACTN2_HUMAN
Specificity	This kit recognizes Human ACTN2 in samples. No significant cross-reactivity or interference between Human ACTN2 and analogues was observed.
Sample Type	Serum, plasma and other biological fluids

Tissue Specificity	Expressed in both skeletal and cardiac muscle.
Post Translational Modifications	Ubiquitinated by FBXL22, leading to proteasomal degradation.
Function	F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Protein Name	Alpha-Actinin-2 Alpha-Actinin Skeletal Muscle Isoform 2 F-Actin Cross-Linking Protein
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-373753 Reactome: R-HSA-390522 Reactome: R-HSA-438066 Reactome: R-HSA-442982 Reactome: R-HSA-5673001 Reactome: R-HSA-9609736 Reactome: R-HSA-9617324 Reactome: R-HSA-9620244
Cellular Localisation	Cytoplasm Myofibril Sarcomere Z Line Colocalizes With Myoz1 And Flnc At The Z-Lines Of Skeletal Muscle
Alternative CLIA Names	Alpha-Actinin-2 CLIA kit Alpha-Actinin Skeletal Muscle Isoform 2 CLIA kit F-Actin Cross-Linking Protein CLIA kit ACTN2 CLIA kit
Specificity	This kit recognizes Human ACTN2 in samples. No significant cross-reactivity or interference between Human ACTN2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item	Specifications	Storage
Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

	Intra-assay Precision	Intra-assay Precision	Intra-assay Precision	Inter-assay Precision	Inter-assay Precision	Inter-assay Precision
Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	255.15	495.46	1831.61	235.73	520.10	1693.41
Standard deviation	32.07	41.72	152.76	20.58	40.10	187.46
CV (%)	12.57	8.42	8.34	8.73	7.71	11.07