• The recombinant ACE2-Fc protein migrates as 120kDa due to glycosylation
  • Recombinant ACE2-Fc functional ELISA assay binding immobilized nCov Spike S1 protein

Human ACE-2 protein (Recombinant) (Fc-Tag) (STJP000681)

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STJP000681

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Host: HEK293
Note: STRICTLY FOR FURTHER RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Recombinant-Human ACE-2-Fc-Tag protein was developed from hek293 and has a target region of Fc-Tag. For use in research applications.
Conjugation: Unconjugated
Formulation: Lyophilised from 0.2 µm filtered PBS solution pH7.4.
Dilution Range: Spin the vial and reconstite in distilled water to a concentration not less than 0.1 mg/mL. This can then be diluted into other buffers.
Storage Instruction: The lyophilized protein is stable for at least 1 year from date of receipt at-20°C. NA
Endotoxin: Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg (1EU/µg). NA
Gene Symbol: ACE2
Gene ID: 59272
Uniprot ID: ACE2_HUMAN
Immunogen Region: ECD
Immunogen: DNA sequence encoding the extracellular domain of Human ACE2 including a C-terminal human IgG1-Fc tag was expressed in HEK293 Cells. NA
Immunogen Sequence: DLFYQSSLAS WNYNTNITEE NVQNMNNAGD KWSAFLKEQS TLAQMYPLQE IQNLTVKLQL QALQQNGSSV LSEDKSKRLN TILNTMSTIY STGKVCNPDN PQECLLLEPG LNEIMANSLD YNERLWAWES WRSEVGKQLR PLYEEYVVLK NEMARANHYE DYGDYWRGDY EVNGVDGYDY SRGQLIEDVE HTFEEIKPLY EHLHAYVRAK LMNAYPSYIS PIGCLPAH
Tissue Specificity Expressed in endothelial cells from small and large arteries, and in arterial smooth muscle cells (at protein level). Expressed in enterocytes of the small intestine, Leydig cells and Sertoli cells (at protein level). Expressed in the renal proximal tubule and the small intestine (at protein level). Expressed in heart, kidney, testis, and gastrointestinal system (at protein level). In lung, expressed at low levels in some alveolar type 2 cells, the expression seems to be individual-specific (at protein level). Expressed in nasal epithelial cells (at protein level). Coexpressed with TMPRSS2 within some lung alveolar type 2 cells, ileal absorptive enterocytes, intestinal epithelial cells, cornea, gallbladder and nasal goblet secretory cells. Coexpressed with TMPRSS4 within mature enterocytes. Isoform 2: Expressed in nasal and bronchial epithelial cells (at protein level).
Post Translational Modifications N-glycosylation on Asn-90 may limit SARS infectivity. Proteolytic cleavage by ADAM17 generates a secreted form. Also cleaved by serine proteases: TMPRSS2, TMPRSS11D and HPN/TMPRSS1. Phosphorylated. Phosphorylation at Tyr-781 probably inhibits interaction with AP2M1 and enables interactions with proteins containing SH2 domains.
Function Essential counter-regulatory carboxypeptidase of the renin-angiotensin hormone system that is a critical regulator of blood volume, systemic vascular resistance, and thus cardiovascular homeostasis. Converts angiotensin I to angiotensin 1-9, a nine-amino acid peptide with anti-hypertrophic effects in cardiomyocytes, and angiotensin II to angiotensin 1-7, which then acts as a beneficial vasodilator and anti-proliferation agent, counterbalancing the actions of the vasoconstrictor angiotensin II. Also removes the C-terminal residue from three other vasoactive peptides, neurotensin, kinetensin, and des-Arg bradykinin, but is not active on bradykinin. Also cleaves other biological peptides, such as apelins (apelin-13, Pyr1apelin-13, apelin-17, apelin-36), casomorphins (beta-casomorphin-7, neocasomorphin) and dynorphin A with high efficiency. In addition, ACE2 C-terminus is homologous to collectrin and is responsible for the trafficking of the neutral amino acid transporter SL6A19 to the plasma membrane of gut epithelial cells via direct interaction, regulating its expression on the cell surface and its catalytic activity. (Microbial infection) Acts as a receptor for human coronaviruses SARS-CoV and SARS-CoV-2, as well as human coronavirus NL63/HCoV-NL63. Isoform 2: Non-functional as a carboxypeptidase. Isoform 2: (Microbial infection) Non-functional as a receptor for human coronavirus SARS-CoV-2.
Protein Name Angiotensin-Converting Enzyme 2
Angiotensin-Converting Enzyme Homolog
Aceh
Angiotensin-Converting Enzyme-Related Carboxypeptidase
Ace-Related Carboxypeptidase
Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2
Database Links Reactome: R-HSA-2022377
Reactome: R-HSA-9678110
Reactome: R-HSA-9679191
Reactome: R-HSA-9694614
Reactome: R-HSA-9733458
Cellular Localisation Processed Angiotensin-Converting Enzyme 2: Secreted
Cell Membrane
Single-Pass Type I Membrane Protein
Cytoplasm
Cell Projection
Cilium
Apical Cell Membrane
Detected In Both Cell Membrane And Cytoplasm In Neurons
Isoform 2: Apical Cell Membrane
Alternative Protein Names Angiotensin-Converting Enzyme 2 protein
Angiotensin-Converting Enzyme Homolog protein
Aceh protein
Angiotensin-Converting Enzyme-Related Carboxypeptidase protein
Ace-Related Carboxypeptidase protein
Metalloprotease Mprot15 Cleaved Into - Processed Angiotensin-Converting Enzyme 2 protein
ACE2 protein
UNQ868 protein
PRO1885 protein

Information sourced from Uniprot.org

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