Human 17 beta-HSD8/HSD17B8 protein (Recombinant) (His-Tag) (STJP017138)

SPECIFICATIONS
HostE.coli
ImmunogenHuman
STJP017138
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General Information

Short DescriptionRecombinant-Human 17 beta-HSD8/HSD17B8-His-Tag protein was developed from e.coli and has a target region of His-Tag. For use in research applications.
ApplicationsSDS-PAGE
HostE.coli
NoteSTRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.

Product Properties

Concentration0.5 mg/mL
FormulationLiquid in 20mM Tris-HCl buffer (pH 8.0) containing 40% Glycerol, 150mM NaCl
Storage InstructionFor short term storage, keep at +2C to +8C for up to 1 week. For long term storage, aliquot and store at-20C, and avoid repeat freeze-thaw cycles.

Target Information

Gene SymbolHSD17B8
Gene ID7923
Uniprot IDDHB8_HUMAN
Accession NumberNP_055049.1
ImmunogenHuman
Immunogen Region1-261aa
Immunogen Sequence MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAA CDLDRAAAQE TVRLLGGPGS KEGPPRGNHA AFQADVSEAR AARCLLEQVQ ACFSRPPSVV VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS IINISSIVGK VGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG FIATPM

Additional Info

Tissue Specificity Widely expressed, particularly abundant in prostate, placenta and kidney. Expressed at protein level in various tissues like brain, cerebellum, heart, lung, kidney, ovary, testis, adrenals and prostate.
Function Required for the solubility and assembly of the heterotetramer 3-ketoacyl-acyl carrier protein (ACP) reductase functional complex (KAR or KAR1) that forms part of the mitochondrial fatty acid synthase (mtFAS). Alpha-subunit of the KAR complex that acts as a scaffold protein required for the stability of carbonyl reductase type-4 (CBR4, beta-subunit of the KAR complex) and for its 3-ketoacyl-ACP reductase activity, thereby participating in mitochondrial fatty acid biosynthesis. Catalyzes the NAD-dependent conversion of (3R)-3-hydroxyacyl-CoA into 3-ketoacyl-CoA (3-oxoacyl-CoA) with no chain length preference.this enzymatic activity is not needed for the KAR function. Prefers (3R)-3-hydroxyacyl-CoA over (3S)-3-hydroxyacyl-CoA and displays enzymatic activity only in the presence of NAD(+). Cooperates with enoyl-CoA hydratase 1 in mitochondria, together they constitute an alternative route to the auxiliary enzyme pathways for the breakdown of Z-PUFA (cis polyunsaturated fatty acid) enoyl-esters (Probable). NAD-dependent 17-beta-hydroxysteroid dehydrogenase with highest activity towards estradiol (17beta-estradiol or E2). Has very low activity towards testosterone and dihydrotestosterone (17beta-hydroxy-5alpha-androstan-3-one). Primarily an oxidative enzyme, it can switch to a reductive mode determined in the appropriate physiologic milieu and catalyze the reduction of estrone (E1) to form biologically active 17beta-estradiol.
Protein Name (3r-3-Hydroxyacyl-Coa Dehydrogenase
17-Beta-Hydroxysteroid Dehydrogenase 8
17-Beta-Hsd 8
Hsd17b8
3-Ketoacyl-Acyl-Carrier-Protein Reductase Alpha Subunit
Kar Alpha Subunit
3-Oxoacyl-Acyl-Carrier-Protein Reductase
Estradiol 17-Beta-Dehydrogenase 8
Protein Ke6
Ke6
Short Chain Dehydrogenase/Reductase Family 30c Member 1
Testosterone 17-Beta-Dehydrogenase 8
Database Links Reactome: R-HSA-75105
Cellular Localisation Mitochondrion Matrix
Alternative Protein Names (3r-3-Hydroxyacyl-Coa Dehydrogenase protein
17-Beta-Hydroxysteroid Dehydrogenase 8 protein
17-Beta-Hsd 8 protein
Hsd17b8 protein
3-Ketoacyl-Acyl-Carrier-Protein Reductase Alpha Subunit protein
Kar Alpha Subunit protein
3-Oxoacyl-Acyl-Carrier-Protein Reductase protein
Estradiol 17-Beta-Dehydrogenase 8 protein
Protein Ke6 protein
Ke6 protein
Short Chain Dehydrogenase/Reductase Family 30c Member 1 protein
Testosterone 17-Beta-Dehydrogenase 8 protein
HSD17B8 protein
FABGL protein
HKE6 protein
RING2 protein
SDR30C1 protein

Information sourced from Uniprot.org

Citations

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